Genetic Diversity in the Vidharbha Region’s Pink Bollworm Pectinophora gossypiella (Saunders) Adults Populations

Author: Lalsingh Rathod, S.K. Bhalkare, P.K. Rathod, A.K. Sadavarte, A.V. Kolhe, D.B. Undirwade, S.S. Mane, Shoba and Chinna Babu Naik

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Abstract

Pink bollworm is the most damaging and ubiquitous cotton pest. Recently "survival of pink bollworm" on bt cotton and causing up to 70-80% losses was reported. Genetic diversity is the result of recombination of genetic material during the inheritance process it shifts over time and space. Individuals' physical characteristics and ability to adapt to stress, diseases, and unfavourable environmental conditions are influenced by genetic diversity. Natural or anthropogenic environmental changes result in natural selection and survival of the fittest. As a result of genetic diversity, susceptible varieties die while those that can adapt to changes survive. In order to understand the variation at genetic level, present experiment "Molecular characterization of pink bollworm adults" has done at Department of Agricultural Entomology and Botany, Centre of Excellence in Plant Biotechnology, Dr PDKV Akola. During the study total of eight primers i.e. seven SSR and one ISSR were used for molecular characterization of pink bollworm adults, collected from different locations viz., Akola, Amravati, Washim, Nagpur, Yavatmal and Wardha of Vidarbha region. Results revealed that a total of 56 amplicons were produced from the molecular characterization of adult pink bollworms with seven SSR and an ISSR primer, of which 31 (55.35%) are polymorphic bands, showing substantial genetic variability in the Pectinophora gossypiella population. The pink bollworm samples were grouped into four major clusters the first group is named as cluster-A, which includes samples belonging to Washim, Nagpur and Yavatmal. Second group is named cluster-B, which includes sample belonging to Amravati. Third group is named cluster-C, which includes samples belonging to Wardha and the fourth group is named cluster-D includes sample belonging to Akola. In the phylogenetic tree, the Washim sample had a higher similarity coefficient (0.774), whereas the Akola sample had a lower similarity coefficient (0.354). It reveals that the Washim sample is highly similar to the Nagpur sample, whereas the Akola sample is the least similar to the Washim and Nagpur samples.

Keywords

Pink bollworm, SSR and ISSR Markers, Molecular characterization, Genetic polymorphism

Conclusion

Molecular characterization of Vidharbha region’s Pink bollworm Pectinophora gossypiella (Saunders) adults populations concludes that at the genetic level Washim sample were highly similar to Nagpur sample whereas, the Akola sample where least similar to Washim and Nagpur sample.

References

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How to cite this article

Lalsingh Rathod, S.K. Bhalkare, P.K. Rathod, A.K. Sadavarte, A.V. Kolhe, D.B. Undirwade, S.S. Mane, Shoba and Chinna Babu Naik (2022). Genetic Diversity in the Vidharbha region’s Pink Bollworm Pectinophora gossypiella (Saunders) Adults Populations. Biological Forum – An International Journal, 14(4): 1137-1140.