Andrographolide from the Methanol Fraction of Andrographis paniculata Leaves: Isolation and Characterization

Author: Anand Shriram Baley and Tarkeshwar Prasad Shukla

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Abstract

The medicinal plant Andrographis paniculata, often referred to as kalmegh, is used in many different cultures for pain relieving of stomach, symptoms of fever, gallbladder problems, intestinal worms, treatment of liver problems functioning, weakness. The leaf is a component of several patented Indian herbal proprietary medicines used to treat liver problems, such as Kalmeghasava and Kalmeghnamay Haub. Diterpenoids, flavonoids, and polyphenols make up its main chemical components. According to reports, the plant's andrographolide, neoandrographolide, and kalmeghnin constituents are its active ingredients. Andrographanin, andrograpanoside, 14-deoxy-12-methoxyandrographolide, and deoxyandrographolide are a few of the other chemical components. Extraction of pure andrographolide from methanol fraction was the main challenge of the research study. This study's goal was to separate andrographolide from A. paniculata leaves and analyze the bioactive components of the methanol fraction. Using column chromatography and gradient elution with several mobile phases, the isolation was carried out. The isolated chemical was analyzed using spectroscopy. Spectral analysis was used as the foundation for the structure elucidation process (UV, IR, 1H NMR, and MASS). The extracted substance from the methanol fraction of leaves was identified as andrographolide based on its spectral properties. This is the first such account of an andrographolide chemical found in the methanol fraction of A. paniculata leaves that has potential as a treatment for a number of ailments.

Keywords

Andrographis paniculata, Isolation, Andrographolide, Acanthaceae, Spectral analysis

Conclusion

The methanol fraction of the leaves of the acanthaceae family plant A. paniculata was successfully used for the phytochemical analysis. Physical, chemical, and spectral data allowed for the compound's identification as andrographolide. The biological activity displayed by the plant's methanolic fraction must be attributed to the andrographolide extracted from this fraction. As a result, it is now up to the pharmacologists and biologists to conduct individual bioactivity tests on the andrographolide to learn more about the plant. Therefore, the current research will encourage scientific communities to conduct additional research on this significant medicinal plant in the near future.

References

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How to cite this article

Anand Shriram Baley and Tarkeshwar Prasad Shukla (2023). Andrographolide from the Methanol Fraction of Andrographis Paniculata Leaves: Isolation and Characterization. Biological Forum – An International Journal, 15(1): 101-106.