Evaluation of Analgesic Activity of Ethanolic, Hydroethanolic, Aqueous and Chloroform Extracts of Nyctanthes arbortristis Leaves

Author: Debnath S., Hazarika A. and Sarma J.

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Abstract

Allopathic antibiotic and anthelmintic overuse leads to the development of antimicrobial and anthelmintic resistance in both humans and animals, while employing plants for treatment that have phytotherapeutic characteristics reduces the likelihood of developing such resistance. Historically, medicinal plants have been used to cure a variety of conditions. Active compounds that are sources of healing can be found in medicinal plants. The current study sought to assess the analgesic potential of a leaf extract from Nyctanthes arbor-tristis. Melonex and tramadol hydrochloride were employed as standard drug for Eddy's hot plate, tail clip, and acetic acid-induced writhing methods of evaluating analgesic activity. Eddy's hot plate, tail clip, and acetic acid-induced writhing procedures were used to test all four extracts (ethanolic, hydroethanolic, aqueous, and chloroform) of Nyctanthes arbor-tristis at doses of 250, 500, and 1000 mg/kg.

Keywords

Nyctanthes arbor-tristis, Analgesic, Eddy’s hot plate method, Tail clip method, Acetic acid induced writhing method

Conclusion

Ethanolic, hydroethanolic, aqueous and chloroform leaf extracts of Nyctanthes arbor-tristis was found to possess significant narcotic analgesic activity when tested by eddy’s hot plate and tail clip method. The four leaf extracts was also found to possess significant non-narcotic analgesic activity in Acetic acid induced writhing test. Among all the four extracts (i.e. ethanolic, hydroethanolic, aqueous and chloroform extracts) of Nyctanthes arbor-tristis under study. Hydroethanolic extract @ 1000mg/kg body weight showed better analgesic activity in comparison to other three extracts in the present study. It may be due to presence of flavonoids and steroids in the extract which inhibit prostaglandin synthesis. The present study indicated that leaves of Nyctanthes arbor-tristis can be used as an alternative to analgesic drug. However further studies are necessary to isolate active ingredients responsible for therapeutic effect and dose determination.

References

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How to cite this article

Debnath S., Hazarika A. and Sarma J. (2023). Evaluation of Analgesic Activity of Ethanolic, Hydroethanolic, Aqueous and Chloroform Extracts of Nyctanthes arbortristis Leaves. Biological Forum – An International Journal, 15(1): 107-118.