In Vitro Apoptosis Detection of MCF-7 and HCT116 Cells by Aqueous Plant Extract from Stachytarpheta mutabilis using PI Annexin V-FITC Flow Cytometry Assay

Author: Ramesh Londonkar and Maithilee Kesralikar

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Abstract

Cancer is one of the main global causes of mortality. Currently available treatment options include chemotherapy, radiation therapy, and surgery, all of which have undesirable side effects. In order to alleviate the negative effects of these therapies, combinational or alternative treatments derived from natural sources are required. Cancer chemopreventive potential of natural plant compounds with significant growth inhibition and apoptosis induction characteristics is being intensively researched. Extracts of plants are an abundant source of secondary metabolites. Stachytarpheta mutabilis is a member of the verbenaceae family and has been used as a folk remedy for ages. It has numerous pharmacological effects, but its anti-cancer potential is the most intriguing. This research addresses the induction of apoptosis in MCF-7 and HCT-116 cell lines after treatment with the aqueous fractions-isolated chemical. Apoptosis is a cell death process marked by distinct morphological and biochemical characteristics. A crucial step of apoptosis is the development of surface modifications by dying cells, which ultimately leads to their identification and ingestion by phagocytes. By coupling FITC (fluorescein isothiocyanate) to Annexin V, flow cytometry may be used to detect and quantify apoptotic cells on a single-cell basis. Our findings show that the anticancer effects of S. mutabilis on human MCF-7 and HCT-116 cell lines are promising. However, further study is required in light of its medicinal potential.

Keywords

Phytochemical analysis. Apoptosis, flow cytometry, Chromatography, MTT assay, anticancer

Conclusion

The findings of this investigation show that the aqueous extract of S. mutabilis might be a promising anticancer drug. Apoptosis is thus valuable as a marker for measuring the efficiency of potential cancer prevention therapy. Following therapeutic treatments, assessing viable and apoptotic cells is facilitated by cell-based assays (Vikas and Anil 2019). By assessing the efficiency of chemicals in vitro, it is possible to find prospective drugs for the treatment of diseases. Using cell-based tests, the therapeutic effectiveness of newly discovered drugs and biologics for the treatment of cancer may be evaluated. The findings of this investigation demonstrate conclusively that the extract was able to suppress cancer cell proliferation by inducing apoptosis. The intriguing finding indicates that the extract may include bioactive chemicals with anticancer properties that need isolation and further characterisation.

References

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How to cite this article

Ramesh Londonkar and Maithilee Kesralikar (2023). In Vitro Apoptosis Detection of MCF-7 and HCT116 Cells by Aqueous Plant Extract from Stachytarpheta mutabilis using PI Annexin V-FITC Flow Cytometry Assay. Biological Forum – An International Journal, 15(1): 139-144.