Formulation Development and Evaluation of Herbal Cream for Candidiasis

Author: Shilpa Thakur and Sharav Desai

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Abstract

The present work describes the formulation and evaluation of topical herbal cream prepared from the leaf extract of Camellia sinensis effective against the yeast Candida albicans. The physicochemical and phytochemical screening was performed for the extract along with antifungal activity and minimum inhibitory concentration. The herbal cream was optimized using different concentrations of white petrolatum and lanolin as independent variables; Spreadability, viscosity, zone of inhibition, and % drug release as dependent variables using 32 factorial designs. The optimized formula was evaluated by different parameters like anticandidal activity, pH, Spread ability, viscosity, homogeneity, consistency, acid value, saponification value, thixotropy study, in vitro drug release study, comparison with marketed formulation, skin irritation study, and stability study. FTIR study showed compatibility of excipients with the extract. The optimized formulation was found brown, having a characteristic odor, pH 6.7, zone of inhibition was 14.96 ± 0.11 mm, Spreadability 15.13 gm.cm/sec, viscosity 1556 cps, homogenous, consistency 5.5 mm, acid value 0.66 and saponification value 185. The drug release was found to be 84.41 % over 180 minutes. Further, an animal study showed no skin irritation, and the prepared formulation was found to be stable. Camellia sinensis extract showed good antifungal activity against the yeast Candida albicans.

Keywords

Camellia sinensis, Candida albicans, Candidiasis, herbal cream, herbal formulation

Conclusion

A herbal formulation was prepared using Camellia sinensis L. for the treatment of Candidiasis. A reformulation study of physicochemical and phytochemical screening was carried out and the results obtained were satisfactory. The standard calibration curve was taken in water and phosphate buffer pH 7.4 at λmax 274 nm. The optimized formulation has pH 6.7, a Spreadability of 15.13 gm.cm/sec, a viscosity of 1556 cps, 14.96 ± 0.11 mm zone of inhibition, and 84.41 % drug release. Skin irritation study on rabbits showed no irritancy explaining that the formulation shows no sensitivity to skin tissues. The short-term stability study showed no changes in the physicochemical properties of formulation during 1 month.

References

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How to cite this article

Shilpa Thakur and Sharav Desai (2023). Formulation Development and Evaluation of Herbal Cream for Candidiasis. Biological Forum – An International Journal, 15(2): 01-08.