Review on: CRISPR-Cas 9: A Novel Genome Editing Tool for Insect Pest Management

Author: Rehana Akbar, Sajad Mohi-ud-din, Summira Rafiq, Raheeba Tun Nisa, Mohmmad Asif Sheikh, Amreena Akhter, Iram Iqbal and Ulfat Jan

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Abstract

Insect pests pose a severe danger to crop productivity, with insects destroying over 40% of crops globally each year. Pesticides used indiscriminately resulted in insect resistance, a reduction in beneficial insect populations, and a slew of other negative consequences for humans and the environment. In addition, the development of transgenic crops has raised ethical concerns, prompting researchers to consider other approaches. The recent advancement of gene-editing technologies such as the CRISPR-CAS9 system has opened up new possibilities for the creation of novel pest control strategy. This review will provide knowledge about efficiency of CRISPR/CAS9 and also some research ideas for CRISPR/CAS9- based integrated pest management. Due to its specificity and trouble-free handling it has been used to a large range of organisms for various research purposes .CRISPR is a technique for editing parts of the genome by removing or modifying DNA. The CRISPR Cas technique functions through a ribonucleoprotein complex, in which Cas9's target recognition lobe interacts with homologous sgRNA to direct specific binding to target DNA, and the excision lobe breaks the DNA. Genome editing's potential has opened up a world of possibilities for designing various features in plants and insects. Genome editing is used to develop pest resistant crops by changing the effector–target relationship and knocking out host susceptibility genes, whereas in insects genome editing is done by knocking out genes responsible for insecticide resistance.

Keywords

Insect Pest, Susceptible Genes, Management, Genome Editing

Conclusion

Crispr/cas technology has exploded in popularity due to its ease of use, low cost, and versatility as a genome editing tool. The CRISPR Cas-9 system is a flexible technology that has already sparked new uses in biology and other domains. This technology has the potential to transform the pace and direction of agricultural research since it allows scientists to precisely and swiftly implant desired features into plants. Crop protection through genetic modification offers a promising alternative to reducing pesticide use that has no discernible impact on human health or the environment. In the future, researchers will focus on using genome editing technologies to improve agricultural productivity, nutritional value, insect pest and disease resistance, and other features. Much research is still needed on its use in agriculture with the aim to eventually use this technology under field conditions for management of insect pest and diseases.

References

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How to cite this article

Rehana Akbar, Sajad Mohi-ud-din, Summira Rafiq, Raheeba Tun Nisa, Mohmmad Asif Sheikh, Amreena Akhter, Iram Iqbal and Ulfat Jan (2023). Review on: CRISPR-Cas 9: A Novel Genome Editing Tool for Insect Pest Management. Biological Forum – An International Journal, 15(1): 550-553.