Antimicrobial Susceptibility of Streptomyces rochei (OM746935) Isolated from Bargur Hills, and their characterization of Bioactive Secondary Metabolites

Author: Dhanasekaran Kokila, Venkatapathi Bhuvaneswari, Manickam Ramesh, Natarajan Ranjani and Ranganathan Kannan

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Abstract

The pursuit of novel bioactive substances from the natural environment has been increasing urgency for reduction of microbial infections. Actinomycetes were isolated from 10 different regions of soil samples in the Bargur hills, from Erode, Tamil Nadu, India. The most potent bioactive compounds producing Streptomyces rochei BF3A strain isolated in the 6th region revealed maximum antibacterial activity against all the tested bacteria viz., gram-positive Staphylococcus aureus (ATCC 29737), Bacillus coagulans (MTCC 6735) and gram-negative bacteria Escherichia coli (ATCC 8739), Klebsiella pneumoniae (ATCC 9621), Pseudomonas aeruginosa (ATCC 27853). The production of bioactive metabolites was extracted using ethyl acetate and the extract showed promising antibacterial activity. The Streptomyces rochei BF3A extract revealed minimum inhibitory concentration (MIC) in all the tested bacteria Staphylococcus aureus- 1.25 mg/ml, Bacillus coagulans – 2.50 mg/ml, Escherichia coli – 2.50 mg/ml, Klebsiella pneumoniae – 2.50 mg/ml, Pseudomonas aeruginosa – 5 mg/ml. The bioactive compounds were partially purified by Thin layer chromatography (TLC) and column chromatography and characterized by gas chromatography-mass spectrometry (GC-MS) and Fourier transformed infrared (FTIR) studies conformed Streptomyces rochei strain active fraction showed predominant bioactive compounds Hexahydro-3-(1-methyl propyl) pyrrolo [1,2-a] pyrazine-1,4-dione, Pyrrolo [1,2-a] pyrazine-1,4-dione hexahydro-3-(2-methyl propyl). These findings demonstrated that the existence of bioactive components in BF3A offered a potential source for the creation of powerful antibacterial drugs that were effective against a variety of bacterial infections. As a result, Actinomycetes must be considered as a unique treatment that requires further research in order to prevent the development of antibiotic-resistant microbes.

Keywords

Bioautography, Fourier Transmission Infra-Red Spectroscopy, Gas Chromatography-Mass Spectrum, Secondary metabolites, Streptomyces rochei

Conclusion

Screening of actinomycetes from Bargur forest yielded 30 actinomycetes from 10 different regions, 6th region of the soil sample exhibited Streptomyces rochei as potent antibacterial activity against all the tested organisms. Bioactive metabolites were separated by TLC and followed by bioautography. The compounds were characterized by FTIR and GC-MS. The major compound was identified as Hexahydro-3-(1-methyl propyl) pyrrolo [1,2-a] pyrazine-1,4-dione. This compound may responsible for the inhibition of bacterial growth with a lower concentration of 1.25 mg/ml. The purification and structural prediction of compounds will be studied in the future.

References

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How to cite this article

Dhanasekaran Kokila, Venkatapathi Bhuvaneswari, Manickam Ramesh, Natarajan Ranjani and Ranganathan Kannan (2023). Antimicrobial Susceptibility of Streptomyces rochei (OM746935) Isolated from Bargur Hills, and their characterization of Bioactive Secondary Metabolites. Biological Forum – An International Journal, 15(1): 564-575.