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Survival and Bio-preservation Studies of Lactic Acid Bacteria in Different Bedding Material

Author: Bharath Kumar N., Suvarna V.C., Girish H.C. and Rashmi S.

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Abstract

Lactic acid (LA) bacterial viability on a bedding material is dependent on temperature, humidity, the composition of the material, light and other physical factors. The present study deals with the viability of a potent lactic acid bacterial consortium in different bedding materials and bio-preservation ability of consortium on onion. The sawdust inoculated LA bacterial consortium had the highest population (128.66 × 103 CFU /g) on 45th day and pH of coir pith treated with consortium was found to be the lowest (2.58) on 45th day. The onion bulbs were artificially inoculated with spoilage organisms and LA bacterial consortium using the pinprick method. The onion bulbs treated with consortium had the least weight loss (8.28 %), no spoilage, the least decrease in pH (5.39) and TSS (4.0) at the end of 28th day of storage. Hence, sawdust as the bedding material inoculated with LA bacterial consortium can be used as an effective bio-protectant.

Keywords

Lactic acid bacteria, Consortium, Bedding material, Pinprick method, pH, TSS

Conclusion

In the present study, the lactic acid bacterial consortium survivability was best in sawdust bedding material and was able to maintain a sufficient population up to 45th day. The pH of the sawdust material decreased moderately in comparison with other bedding material. LA bacterial consortium increased the shelf life of onion for 28 days after the artificial inoculation of spoilage organisms. Further research can be taken up on the probiotic properties of LA bacterial consortium, molecular identification of genes involved in antimicrobial compound production.

References

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How to cite this article

Bharath Kumar N., Suvarna V.C., Girish H.C. and Rashmi S. (2023). Survival and Bio-preservation Studies of Lactic Acid Bacteria in different Bedding Material. Biological Forum – An International Journal, 15(1): 588-591.