DNA Fingerprinting of Sugarcane Genotypes/Varieties for Molecular Evidence and Protection

Author: Niraj Nath Tiwari, Sujeet Pratap Singh, Sachin Kashyap and Arvind Kumar

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Abstract

DNA fingerprint databases are vital and imperative tools for plant molecular research because they provide potent information for crop breeding, variety quality control, variety right protection, and molecular marker-assisted breeding. Morphological descriptors may also use for physical fingerprinting but the reduced number of descriptors makes it difficult to identify the large number of varieties. DNA fingerprinting of seventeen sugarcane (Saccharum spp.) genotypes were constructed with three SSR primers were found polymorphic and gave clear bands. Sugarcane genotypes of both commercial hybrids and genetic stocks commonly used as parents in breeding programme were included in the study. A total of 157 markers were amplified, of which 68.0% were polymorphic with an average of ~23% polymorphic bands per primer. Number of bands produced by various primers was ranged from 4 to 12 with an average of 5.3 fragments per primer. DNA fragments size were ranged between 147 to 1474 bp. Analyses of molecular profiles generated by SSR markers revealed the unique bands specific for genotypes. The all used sugarcane genotypes/varieties were identified by either presence or absence of specific DNA fragments. Primers used in this experiment were able to distinguish all genotypes of sugarcane, demonstrating the ability of the methodology to determine unique genetic profiles. The identified unique markers could be useful for identification, utilization and management of the genotypes.

Keywords

Sugarcane, DNA Fingerprinting, Genotypes, Varieties, SSR Markers

Conclusion

SSR based DNA fingerprinting was used to develop 157 markers for genotypes/varieties identification and fingerprint construction. The conclusion of this study provides a useful approach for genotyping, classifying, and identifying genotypes/varieties and resources in sugarcane breeding. The identified unique markers could be useful for identification, utilization and management of the genotypes.

References

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How to cite this article

Niraj Nath Tiwari, Sujeet Pratap Singh, Sachin Kashyap and Arvind Kumar (2023). DNA Fingerprinting of Sugarcane Genotypes/verities for Molecular Evidence and Protection. Biological Forum – An International Journal, 15(2): 86-88.