Decolorization Efficiency of Crude Laccase Extract of Cladosporium magnoliigena LACF6 on different Textile Dyes

Author: Jaya Dayal*, Anuradha Singh and Nupur Mathur

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Abstract

Many industries use synthetic dyes, but their use pollutes the environment and endangers the lives of people., Despite its high color concentrations, only a tiny percentage of textile effluent is treated. They pollute waterways and harm terrestrial and aquatic ecosystems. Therefore, textile dyes must be removed from wastewater before their final discharge into the receiving water bodies. However, their removal from wastewater remains a significant challenge due to the stable nature of dyes. These effluents are typically treated using physical and chemical processes, but these methods are costly, complex, and may produce harmful intermediates. Dye decolorization using microorganisms and their enzymatic systems is a method that is both cost-effective and helpful to the environment. It can reduce the pollution caused by the dyeing industries. In this work, crude laccase enzyme from C. magnoliigena LACF6 was extracted. It is found to have good dye removal efficacy for the various dyes used in this investigation. The color removal efficiency of crude laccase extract was around 75.73 ± 3.00 when applied to Victoria Blue B dye at a concentration of 1 mg/ml. However, at the same conditions, the decolorization percentages for Congo Red, Alizarin Red S, and Methylene Blue were 31.69 ± 3.11, 28.30 ± 1.54, and 11.09 ± 1.44, respectively. The significance of crude laccase (C. magnoliigena LACF6) in treating industrial effluents containing dyes is seen in the results of this experiment, which indicate that this crude laccase extract has a high potential for decolorizing various structurally different textile dyes.

Keywords

ABTS, crude enzyme, Dye decolorization, Fungus, Synthetic textile dyes, Tannic acid

Conclusion

One of the principal sources of water contamination in the textile dyeing industrial area is the direct dumping of chemicals, primarily dye-containing effluents, into the open environment. The dyes seriously threaten the ecosystem and negatively affect all living organisms. Therefore, removing dyes and other chemical compounds from industrial effluents prior to their discharge into open water bodies is a key concern. There are various physicochemical and biological techniques for dye treatment. Dye decolorization with microorganisms and their enzymatic system is a cost-effective and environmentally beneficial solution for reducing the pollution from the dye industry. In the current preliminary study, crude laccase enzyme was extracted from C. magnoliigena LACF6 and has been shown to have 12.75 ± 1.47U/ml laccase activity and good color removal efficiency for the several classes of dyes. For example, at a dye concentration of 1 mg/ml, the color removal efficacy of Victoria Blue B dye by crude laccase extract was 75.73 ± 3.00. However, at the same initial dye concentration, the decolorization percentages for Congo Red, Alizarin Red S, and Methylene Blue were 31.69 ± 3.11, 28.30 ± 1.54, and 11.09 ±1.44 respectively, under the same conditions. On the other hand, it has been seen that Trypan Blue, Malachite Green, and Orange G could not be decolorized effectively with this enzymatic treatment. The findings showed that crude laccase from C. magnoliigena LACF6 is a significant biological and eco-friendly approach for treating colored dye wastewater.

References

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How to cite this article

Jaya Dayal, Anuradha Singh and Nupur Mathur (2023). Decolorization Efficiency of Crude Laccase Extract of Cladosporium magnoliigena LACF6 on different Textile Dyes. Biological Forum – An International Journal, 15(2): 213-219.