Influence of various Carbon and Nitrogen Sources on Mycelial Growth of Fusarium oxysporum f. sp. lini

Author: Yashowardhan Singh, Jayant Bhatt, Vijay Kumar Yadav, Sanjay Kharte and Swarna Kurmi

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Abstract

Linseed is the oilseed crop with the greatest omega-3 fatty acid content. It also contains a lot of protein, fiber and 45-50% oil contain. Fusarium oxysporum f. sp. lini causes Fusarium wilts in linseed. This disease is mostly soil-borne, but it may also be seed-borne. In studying the influence of various carbon and nitrogen sources on mycelial growth of Fusarium oxysporum f. sp. lini is the need to control for other factors such as temperature, pH, and light intensity, which can also affect fungal growth. Despite these challenges, research on the influence of carbon and nitrogen sources on fungal growth has contributed significantly to our understanding of fungal physiology and metabolism. By identifying the optimal growth conditions for F. oxysporum f. sp. lini, researchers can improve fungal culture methods and develop more effective strategies for controlling Fusarium wilt disease in linseed plants. In this work, four carbon and seven nitrogen compounds were examined In vitro to determine the nutritional requirements of the linseed wilt fungus Fusarium oxysporum f. sp. lini. Maximum mycelial growth was found in fructose to the influence of different carbon sources on the development of the test pathogen. Fructose followed by D-glactose, Mannital, and Glucose. All of the carbon sources used was superior to control. Potassium nitrate was determined to be the most suitable nitrogen source for growth of F. oxysporum f. sp. lini, followed by ammonium acetate. When compared to the control, the total nutrition considerably affected the development of test fungus under in vitro condition.

Keywords

Fusarium oxysporum f. sp. lini, linseed, carbon and nitrogen, mycelial growth

Conclusion

The mycelial growth of Fusarium oxysporum f. sp. lini showed significant variation when different nitrogen and carbon nutritional sources were used. Fructose, as a carbon source, was shown to be favourable for mycelial growth. Potassium nitrate was shown to be the best nitrogen source for mycelial development. The results show which nutrients can increase the virulence of Fusarium oxysporum f. sp. lini. This study can be further expanded by investigating the effect of different environmental conditions, such as temperature, pH, and light intensity, on the growth and pathogenicity of F. oxysporum f. sp. lini. Additionally, the study can be extended to identify potential antifungal agents that can effectively control Fusarium wilt disease in linseed plants. Such studies can help in the development of more efficient and sustainable strategies for controlling this devastating disease.

References

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How to cite this article

Yashowardhan Singh, Jayant Bhatt, Vijay Kumar Yadav, Sanjay Kharte and Swarna Kurmi (2023). Influence of various Carbon and Nitrogen Sources on Mycelial Growth of Fusarium oxysporum f. sp. lini. Biological Forum – An International Journal, 15(2): 302-307.