Utilization of 30 Cyanobacterial extracts for the Synthesis, Screening and Optimization of AuNPs: A Promising Approach for Green Chemistry

Author: Shehzadi, Nida Asif, Nafe Aziz, Pooja Srivastava, Raj Kumar, Nadeem Ahmad and Tasneem Fatma

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Abstract

Gold nanoparticles (AuNPs) became a wide area of research, due to their unique chemical and physical features they are widely used in biological applications. The challenges of the study was to synthesized less toxic ecofriendly nonhazardous biocompatible nanoparticles. Considerable increasing demand of such AuNPs in different fields, especially in nanomedicines, there is an urgent need to maintain constant supply of top quality biogenic AuNPs. Material methods: 30 cyanobacterial extracts were screened for AuNPs synthesis based on the minimum reduction time and size of NPs. Best strain optimized with set controlled conditions of 1mM HAuCl4, 45 ml of extract volume, pH 6.5, and 60°C. And characterized by UV-Visible, FTIR and AFM characterized. Results: Purple to ruby red of reaction mixture is visual indication of AuNPs, further confirmed by UV–Vis spectroscopy and SEM, FTIR and AFM. During screening Phormidium sp. synthesized spherical smallest AuNPs (08-23 nm) within 35 minutes. Optimization resulted in reduction in size from 08-23 nm to 04-07 nm (SEM) and time 35 to 25 min.

Keywords

Cyanobacteria, Gold nanoparticles (AuNPs); Screening, Optimization, FTIR, AFM

Conclusion

Through present study success was achieved in adding cyanobacteria as a novel source for biogenic AuNPs. All cell extracts (30 cyanobacteria) exhibited color change of reaction mixture from purple to ruby red indicating their potential AuNPs synthesis. Phormidium synthesized smallest 08-23 nm nanoparticles within 35 minutes and appeared as best the strain. Optimization of reaction condition for AuNPs synthesis resulted in reduction of size (08-23 nm to 04-07 nm) and time (35 min to 25 min), that were characterized by UV visible spectroscopy, FTIR and AFM analysis.

References

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How to cite this article

Shehzadi, Nida Asif, Nafe Aziz, Pooja Srivastava, Raj Kumar, Nadeem Ahmad and Tasneem Fatma (2023). Utilization of 30 Cyanobacterial extracts for the Synthesis, Screening and Optimization of AuNPs: A Promising Approach for Green Chemistry. Biological Forum – An International Journal, 15(2): 689-697.