Studies on Gas Chromatography-Mass Spectrometry (GC-MS) and Thin Layer Chromagraphy (TLC) Analysis of Three Spotted Crab (Portunus sanguinolentus)

Author: K. Madhubala and T. Selvamohan

Download PDF

Abstract

Crabs are decapods crustaceans of the family Portunidae. Carbs obtain carotenoids from an external sources like medium and food materials. It has been suggested that carotenoids are strong antioxidants that shield membrane lipids from harmful peroxidation processes. The peculiar spectrum characteristics of carotenoids were frequently used for detection and quantification; these chemicals' conjugated polyene architectures give them their distinct light absorption spectra, high molar absorptivity, and remarkable lower limits of detection. In the present study, carotenoids are extracted from the three spotted crabs. I collected from the South East Coast of India. The results revealed that carotenoid compounds were distributed a varied level in the carapace and muscle. Increasing variability was observed in carotenoid groups between individuals concerning age. The quantities of carotenoids of hexane extract were determined by GC-MS and TLC respectively. The experimental crab species seems to contain a comparable concentrations of carotenoids.

Keywords

Three spotted crab, Carotenoids, FAME, GC-MS, and TLC

Conclusion

Many useful bioactive compounds are found out from the crab shell extract. These compounds are play important role as well as several antimicrobial molecules were detected for further studies.

References

-

How to cite this article

K. Madhubala and T. Selvamohan (2023). Studies on Gas Chromatography-Mass Spectrometry (GC-MS) and Thin Layer Chromagraphy (TLC) Analysis of Three Spotted Crab (Portunus sanguinolentus). Biological Forum – An International Journal, 15(2): 712-718.