LIP1 Regulates Lipid Metabolism in Saccharomyces cerevisiae

Author: Arul Mathivanan and Vasanthi Nachiappan

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Abstract

LIP1 is an acyl-CoA-dependent ceramide synthase vital in synthesizing sphingolipids. However, the function of LIP1 in the other main lipids, such as phospholipids and neutral lipids, is still to be investigated. In this work, we evaluated the influence of the loss of LIP1 on overall lipid metabolism, and we discovered that the deletion of LIP1 had disrupted cell development, neutral lipid metabolism, and phospholipid homeostasis. As adequate phospholipid production is crucial for counteracting ER stress, we subjected the LIP1 deletion cells to ER stress (DTT treatment). We discovered that the LIP1 deletion cells were more vulnerable to ER stress than the wild. The levels of ER stress response genes levels were also elevated in LIP1 deletion cells compared to the control. Thus, our study provides the new function of LIP1 in ER stress response mechanisms and lipid homeostasis.

Keywords

Ceramide, sphingolipids, phospholipids, neutral lipids, ER stress, DTT

Conclusion

Our results conclude that apart from the role of LIP1 in ceramide synthesis, LP1 also plays a significant modulatory role in producing phospholipids and neutral lipids. Phospholipids mainly function as structural lipids, and their synthesis was downregulated, while the TAG and lipid droplets (LD) synthesis was upregulated. Loss of LIP1 severely affected cell growth under ER stress and increased the expression of ER stress response genes, thus implying LIP1’s role in maintaining ER stress and protein quality control.

References

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How to cite this article

Arul Mathivanan and Vasanthi Nachiappan (2023). LIP1 Regulates Lipid Metabolism in Saccharomyces cerevisiae. Biological Forum – An International Journal, 15(2): 737-741.