Screening of Soybean Genotypes against Cowpea Mild Mottle Virus Infection

Author: Manoj Kumar Yadav, Mukesh Kumar Yadav, Kumkum Verma, Shahil Kumar, Abhishek Dubey, Pankhuri Singhal, K.P. Singh, S.K. Lal and V.K. Baranwal

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Abstract

Cowpea mild mottle virus (CPMMV), a whitefly and seed transmitted carla virus, is an emerging soybean disease in India and many tropical and subtropical regions of Africa and Asia. This virus causes significant yield and quality losses under field conditions. The utilization of resistant varieties is the best strategy to manage the losses caused by the CPMMV. Keeping this in mind, a field experiment was conducted at ICAR-IARI, New Delhi, situated at 28.6° N latitude, 77.16° E longitude at an elevation of 228.61 m above the mean sea level. The present study was conducted to identify the soybean genotypes resistant against cowpea mild mottle virus infection. Soybean genotypes with varied symptoms; mild mosaic, systemic mottling, stunting and leaf deformation were observed under field conditions. The CPMMV disease incidence in the soybean field varied from 10-85% during Kharif 2019. The field screening study identified fifty soybean genotypes as resistant to moderately resistant, while 40 genotypes showed susceptible reactions. These fifty genotypes were phenotyped using sap inoculation under controlled conditions. The glasshouse study showed that nine (18%) soybean genotypes showed a moderately-resistant reaction, nine genotypes were moderately susceptible, thirteen genotypes showed a susceptible reaction and 19 genotypes were highly susceptible against CPMMV infection. The current study would help in the utilization of identified genotypes in soybean resistance breeding to develop CPMMV resistant varieties and study host-pathogen interaction.

Keywords

CPMMV, mottle, genotypes, resistance, mechanical inoculation

Conclusion

Field screening complemented with controlled glasshouse screening of soybean genotypes would help in identification of resistant genotypes against CPMMV infection. This would result in their rational use through their utilization in soybean resistant breeding programs, identification of novel gene(s)/allele(s) and study of host pathogen interaction at molecular level.

References

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How to cite this article

Manoj Kumar Yadav, Mukesh Kumar Yadav, Kumkum Verma, Shahil Kumar, Abhishek Dubey, Pankhuri Singhal, K.P. Singh, S.K. Lal and V.K. Baranwal (2023). Screening of Soybean Genotypes Against Cowpea Mild Mottle Virus Infection. Biological Forum – An International Journal, 15(2): 779-783.