Protective Effects of Garcinia talbotii bark extract against Induced Oxidative Damage on Human Erythrocytes

Author: Sandeep Kumar Jain R., Meghana P., Prashanth N., Pooja S. Rajaput and Kumaraswamy H.M.

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Abstract

Garcinia talbotii is a tree belonging to the family Clusiaceae, found in evergreen forests of Western Ghats. Reactive oxygen species induces cell and tissue injury and contribute to chronic inflammation underlying many neurodegenerative, cardiovascular, and metabolic diseases. Shade dried Garcinia talbotii bark was subjected to ethanol extraction using soxhlet apparatus and Garcinia talbotii bark ethanol extract was screened for protective effects against H2O2 induced oxidative damage on human erythrocytes by HRBC membrane stabilization assay, inhibition of albumin denaturation assay and also inhibition of H2O2 induced oxidative damage on erythrocyte ghost membrane proteins by SDS-PAGE. The results showed that Garcinia talbotii bark extract (GBE) at a concentration of 50-250μg/ml, significantly protected the heat induced protein denaturation. At the concentration of 100 and 200 μg/ml, GBE significantly (p<0.01) inhibited H2O2 induced haemolysis and also protected the erythrocyte morphology, which was further confirmed by performing SDS-PAGE for erythrocyte ghost membrane proteins. The present study for the first time demonstrates the in vitro anti-inflammatory effects of Garcinia talbotii.

Keywords

Garcinia talbotii, Erythrocytes, Inflammation, Oxidative damage, Ghost membrane

Conclusion

The presence of bioactive compounds in Garcinia talbotii bark extract contributed to the protection of proteins and HRBC membrane, oxidative stress in erythrocytes has been significantly reduced by Garcinia talbotii bark extract. The results obtained in the present study indicate that ethanol extract of Garcinia talbotii bark can be a potential antioxidant and anti-inflammatory agent.

References

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How to cite this article

Sandeep Kumar Jain R., Meghana P., Prashanth N., Pooja S. Rajaput and Kumaraswamy H.M. (2023). Protective Effects of Garcinia talbotii bark extract Against Induced Oxidative Damage on Human Erythrocytes. Biological Forum – An International Journal, 15(3): 16-20.