Antimicrobial activity of Polyalthia longifolia extract, fungal chitosan and its combinatorial studies

Author: Shankara S., Srinivasulu M.V., Roshan A. and Narasimha Murthy K.

Download PDF

Abstract

Medicinal plants play an important role in protective our health and these plants are nature's gift to lead defensive life. India is one of the greatest ethanoculturally varied nations in the world, where the remedial plant area is part of a time privileged practice that is treasured even today. Pharmacologic research on the plant parts displays effective cytotoxic function, hypotensive effect, hypoglycemic activity, antiulcer activity, and antimicrobial activity. In this present study the antimicrobial activity of combinatorial studies of aqueous extract Polyalthia longifolia and chitosan (FCh) extracted from Aspergillus niger. The extracted FCh characterized by by using UV-visible spectroscopy, FT-IR, and SEM coupled with EDX analysis. Further, the antimicrobial activity of aqueous extract of P. longifolia and chitosan (FCh) was checked by using disc diffusion assay. Also the combinatorial antimicrobial activity studies of FCh and P. longifolia were studied. The results of FT-IR showed, the characteristic peaks of FCh shows at 3435 cm-1, 2924cm-1, 1645 cm-1, 1412 cm-1and 1021 cm-1 attributing towards O-H strech, C-H strech, N-H bend C-H bend-C-N stretch. The characteristic peak at 191 nm obtained from spectrophotometric evaluation confirms the presence of Ch. The microscopic view of FCh was also observed through SEM coupled with EDX showed that FCh smooth surface. The antimicrobial action of aqueous extract of P. longifolia zone of inhibition was ranges from 10.6±0.1 to 12.6±0.3mm and FCh show 11.6±0.5 to 18.2±0.3 mm at 0.5 to 10mg/ml concentrations. The combinatorial studies FCh and P. longifolia showed highest percent mycelial inhibition against A. flavus and F. verticillioides. The percent mycelial inhibition of A. flvaus was 87.6±0.4 % and F. verticillioides 97.6±0.5% at 0.5 to 10mg/ml concentrations. The obtained results confirms that the combinatorial studies FCh and P. longifolia could be used as alternative green strategy for the control of microbial activity.

Keywords

Polyalthia longifolia, chitosan, characterization, antimicrobial activity, mycelial inhibition, combinatorial studies

Conclusion

In this study the chitosan was extracted from A. niger. The yield after 13th day of incubation. The UV-visible spectrophotometer, FTIR, SEM coupled with EDX also confirmed that chitosan. The extracted fungal chitosan was subjected to antimicrobial activity, and it was found the fungal chitosan were active against the bacteria and fungi. Our finding suggests that the A. niger is the potential candidate to produce eco-friendly chitosan in the development of drugs and food industries. Future prospective of the study may be extended in the following future directions. Apart from aqueous extract of P. lonifolia, the major components and its combition of FCh used as antimicrobial agent. Further, the preparation of fungal chitosan and its combination of P. lonifolia can be evaluated at nanolevel. The FCh and its combination of other plant extracts, their components can be studied at nanolevel used as antimicrobial agent.

References

-

How to cite this article

Shankara S., Srinivasulu M.V., Roshan A. and Narasimha Murthy K. (2023). Antimicrobial activity of Polyalthia longifolia, extract fungal chitosan and its combinatorial studies. Biological Forum – An International Journal, 15(2): 836-842.