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Characterization of Black Sesame (Sesamum indicum L.) Genotypes through Chemical Tests

Author: J.G. Savaliya, C.A. Babariya, M.R. Prajapati, M.J. Jadav and R.B. Mori

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Abstract

A knowledge of different genotype is a prerequisite for any successful improvement programme. A study was conducted to characterize 40 black sesame genotypes based on the chemical tests during the summer of 2020 at the Department of Seed Science and Technology, Junagadh Agricultural University. For the purpose of discriminating the genotypes, the seeds were subjected to the NaOH, KOH, seedling growth response to GA3, and 2, 4-D test. The genotypes were divided into three colour categories based on the seed coloration with NaOH: dark brown (12 genotypes), light brown (11 genotypes), and brown (7 genotypes). Genotypes were divided into four categories based on the colour of the solution caused by peroxidase activity: brown (15 genotypes), light brown (9 genotypes), no change (4 genotypes), and dark brown (2 genotypes). None of the examined sesame genotypes could be distinguished using the KOH and NaOH tests. So, this study is helpful for easy identification of genotype based on chemical test which are negate cumbersome morphological identification.

Keywords

Black sesame, Characterization, Chemical test

Conclusion

It can be said that a crucial factor in any programme for producing seeds is the evaluation of genetic purity. Thus, methods for determining genetic purity and characterising varieties must be made simple and trustworthy. The DUS test, seed production programme, and genetic purity testing could all benefit from the identified morphological traits of wheat genotypes. The outcome of a chemical test is helpful for both classifying and identifying wheat genotypes as well as for determining genetic purity.

References

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How to cite this article

J.G. Savaliya, C.A. Babariya, M.R. Prajapati, M.J. Jadav and R.B. Mori (2023). Characterization of Black Sesame (Sesamum indicum L.) Genotypes through Chemical Tests. Biological Forum – An International Journal, 15(2): 916-922.