Author: S. Gini, S. Illanjiam, S. Priya Grace, C. Shanmugasundaram, T. Srinivasan, P. Nirmala, R.V. Prabu, K.N. Gayathri, S.R Sriram Kumar and A. Ganesh Kumar
In this study, microorganisms were isolated and identified from seafood processing effluent from Chennai. Microorganisms were isolated after the samples were serially diluted, inoculated on Nutrient agar media and Sabouraud Dextrose agar, respectively. The spread plate technique was used for the isolation process. The bacterial isolates were described by Gram staining and identification by biochemical assays. The colony and morphology of the isolates were also noted. Wet mount technique with lacto phenol method was used to examine the fungal isolates. According to the results, nine bacterial and four fungal isolates from the effluent were found, with Pseudomonas species being the most prevalent. Staphylococcus aureus, Bacillus species, Micrococcus species, Salmonella species, Vibrio species, Escherichia coli, Klebsiella species, and Shigella species. The isolated and identified fungi are Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Curvularia, Alternaria. The isolates also showed resistant to some antibiotics. These bacteria causes ill effects to humans, so this effluent should be treated properly and treated seafood processing effluent can be used for agricultural purposes. So the study will be helpful to identify and isolate the pathogenic microorganism and also used for the future generation.
Seafood processing Effluents, Bacteria, Fungi, Morphological, Biochemical, Antibiotic Sensitivity
In conclusion, the extensive use of antibiotics in the production and processing of seafood is clearly supported by the large microbial load in the effluent and their resistance to several common antibiotics. If discharged without being properly treated, effluents harboring resistant bacteria may pollute the receiving river and other natural water bodies. As it enters the food chain, seafood taken from contaminated waterways may act as passive carriers of resistance that can harm people. Hence, identifying the risk factors involved in the transmission and spread of drug-resistant bacteria may be aided by early detection, the identification of antibiotic resistance patterns, and the characterization of the isolates from a seafood processing industries. Hence, good waste management procedures in processing facilities would not only preserve seafood cleanliness but also safeguard the environment.
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S. Gini, S. Illanjiam, S. Priya Grace, C. Shanmugasundaram, T. Srinivasan, P. Nirmala, R.V. Prabu, K.N. Gayathri, S.R Sriram Kumar and A. Ganesh Kumar (2023). Microbial Population Characterization Associated with Effluents obtained from Seafood Processing Industries. Biological Forum – An International Journal, 15(2): 948-953.