Analysis of Bioactive Compounds in Methanolic Leaf Extract of Diospyros melanoxylon Roxb. Challenged with AM Fungi by Liquid Chromatography Mass Spectrometry (LC-MS) Technique

Author: S. Syam Prasad and P.R. Sushama

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Abstract

Plants are abundant sources of a variety of bioactive phytochemicals that have a variety of biological impacts. The protection of human health and benefit come from the screening of phytochemical substances. Diospyros melanoxylon (Roxb.), a member of the Ebenaceae family, is a significant commercial, multipurpose, and medical plant utilized in a variety of circumstances. The primary goal of the current experiment was to examine the phytochemical substances in the leaves of the D. melanoxylon challenged with AM fungi using liquid chromatography and mass spectrometry, (LC–MS). Plant challenged with AM fungi leaves samples were analyzed with LC-MS analysis and 27 different phytochemical substances with different molecular weight were found. The primary bioactive substances were gallic acid, ursolic acid, oleanolic acid and betulinic acid (Rt-1.07, 23.82, 23.70 and 24.44). Analysis of the mass spectra has allowed for the identification of the chemicals. The use of D. melanoxylon leaves for treating a number of ailments is confirmed by the presence of several bioactive chemicals with diverse chemical structures. Hence, it is claimed that plant is crucial for pharmaceuticals. Moreover, it might be necessary to isolate specific bioactive substances in order to discover a novel medicine.

Keywords

Diospyros melanoxylon, Leaves, LC–MS, Phytochemicals, Methanol

Conclusion

The compounds identified in the D. melanoxylon leaves extract most have medicinal properties and some have antimicrobial activities. Present study declared that the 27 different phytochemical compounds were identified as the phenolics, flavonoids, terpenoids, fatty acids and some unknown compounds having several enormous applications. Hence it can be used for further processing like encapsulation.

References

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How to cite this article

S. Syam Prasad and P.R. Sushama (2023). Analysis of Bioactive Compounds in Methanolic Leaf Extract of Diospyros melanoxylon Roxb. Challenged with AM Fungi by Liquid Chromatography Mass Spectrometry (LC-MS) Technique. Biological Forum – An International Journal, 15(2): 981-989.