Ameliorative Role of Lactobacillus casei and Bifidobacterium bifidum in Spirotetramat induced Oxidative Stressed Wistar Rats

Author: Shariq Hussain and Madhavi Gaur

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Abstract

Pesticides have created a severe mess throughout the agricultural world by altering various biological processes and researchers are targeting the direct their studies in order to minimize their ill effects by practicing different agents from organic background. Spirotetramat, a tetramic acid derived pesticide has been found to cause a variable toxicity among living organisms at different levels, there by causing an environmental threat to non-target organism. Keeping this challenge in the view we tried to minimize this treat with alternative intake of probiotics. In this study we have tried to find out the ameliorative role of probiotics Lactobacillus casei and Bifidobacterium bifidum in Spirotetramat induced toxicity in Wistar rats. The rats were divided in to five groups with a control group for reference. Toxicity was induced by administration of Spirotetramat at 667mg/kgbw/day for 28 days along with gavaging of probiotics (L. casei and B. bifidum 1 × 107 cfu/ml). Enzymatic activities like were estimated by prescribed protocols for estimation of oxidative stress. Lipid peroxidation (LPO), Superoxide dismutase (SOD), Catalase CAT, Glutathione peroxidase (GPx) and Glutathione (GSH) levels were estimated after sacrifice. Lipid per oxidation (LPO) was found significantly increased (302.5 % and 290.5% in Liver and Kidney respectively) (P<0.01); however in the treated groups with L. casei and B. bifidum showed a significantly (P<0.01) reduction in LPO (53.5%). Glutathione (GSH) was found to decreased significantly (P<0.01) (70.2% and 75.3% in Liver and Kidney respectively) in pesticide administrated rats as compare to untreated ones. Superoxide dismutase (SOD) levels in toxicity induced rats significantly (P < 0.01) decreased (43.5% and 49.5% in liver and kidney respectively) in comparison with untreated rats. Catalase (CAT) was also found to be decreased significantly (P < 0.01) (35.09% and 32.20% in liver and kidney respectively) that was observed in comparison to normal rats. After the treatment with L. casei it resulted in significant (P < 0.05, 43.08%) increase in CAT whereas in combination of both probiotics and B. bifidum separately it showed a significant (P < 0.01) increase in CAT. For Glutathione peroxidase (GPx) levels, a significant (P < 0.01) decrease was reported (71.20% and 67.20% in liver and kidney respectively) when compared to normal rats.

Keywords

Lactobacillus, Oxidative stress, Probiotics, Spirotetramat, Toxicity

Conclusion

By estimating the relative oxidative stress among different groups of Wistar rats, it is finally concluded that probiotics have a promising role in control of organ toxicity and hence can be used as an alternative therapy to minimize the relative ill effects Spirotetramat.

References

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How to cite this article

Shariq Hussain and Madhavi Gaur (2023). Ameliorative Role of Lactobacillus casei and Bifidobacterium bifidum in Spirotetramat induced Oxidative Stressed Wistar Rats. Biological Forum – An International Journal, 15(2): 1038-1042.