Estimation of Total Phenolic and Flavonoid Content and Antioxidant Activity in Catharanthus roseus L. (Alba) Plant Extract

Author: Tolambiya Priyanka, Jagetiya B.L. and Mathur Sujata

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Abstract

The Phenolic compounds are important plant metabolite which have redox properties liable for antioxidant activity. The hydroxyl group of phenolic compounds facilitate free radical scavenging. The Total Phenolic Content of each extract of leaf, stem, root, and flower were determined by the Folin-Ciocalteau method and calculated as Tannic acid equivalents (TAE) g-1. This has also been used to compute antioxidants in multifaceted biological structures. DPPH method is being used to measure the antioxidant activity of methanolic extract. The odd electron in the DPPH free radical gives a strong absorption maximum at 517 nm and gives purple color. These leaf extract have maximum phenolic content 1378 µg and for Flower extract it is 1185 µg from regression curve. The Total flavonoids were found in leaf and Flower extract are 0.60% and 1.70% respectively. The antioxidant activity of leaf extract was derived from a calibration curve (Y=0.2934x + 40.605, R2 = 0.9079) and for flower extract (Y=12.982x – 415.8, R2 = 0.9365) from this calibration curve. So R2 value is maximum for Leaf and Flower extract. The IC50 value of stem sample is 688.47µg/ml therefore inhibition activity or antiradical efficiency is maximum of stem extract of Catharanthus roseus L. (Alba). By comparing the correlation coefficients (R-values), it could be suggested that phenolic and flavonoid groups are highly accountable for the antioxidant activity. This study can also prove by comparing antidiabetic drug metformin and nifedipine, the aqueous extract of the leaves of Catharanthus roseus by conducting experiment on sugar induced wistar rats, Catharanthus roseus leaves extract demonstrate better antioxidant activity in the treatment of diabetes and hypertension.

Keywords

Antioxidant activity, Total phenolic content, Regression curve, IC50 value, Antiradical efficiency, 2, 2-diphenyl- 1-picrylhydrazyl (DPPH), R2

Conclusion

The phenolic and flavonoid groups are highly responsible for the antioxidant activity. So, there is linear correlation between Total Flavonoids, Total phenolic content, and antioxidant activity. In this study, antioxidant activity indicates extract of leaf and flower sample with higher phenolic and flavonoid contents could be a significant source of natural antioxidants. But the IC50 value is less of stem sample. The lower the IC50 value, the more potent is the substance at scavenging DPPH and this indicate higher antioxidant activity.

References

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How to cite this article

Tolambiya Priyanka, Jagetiya B.L., Mathur Sujata (2023). Estimation of Total Phenolic and Flavonoid Content and Antioxidant Activity in Catharanthus roseus L. (Alba) Plant Extract. Biological Forum – An International Journal, 15(2): 1101-1106.