Evaluation of Antioxidant and Antimicrobial Potential of Herbal Phenolics

Author: Jyotsna Anandrao Saonere, Madhuri A. Channawar, Nitin I. Kochar, Deepak Mohale and Anil V. Chandewar

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Abstract

Antimicrobial resistance is the big challenge across the globe. Infectious and non infectious diseases are the consequences of microbial invasion of human biological system. Worldwide infectious diseases are the second leading cause of death and disability. Antimicrobial resistance is the consequence of inappropriate use of antibiotics in noninfectious disease. Herbal bioactives are the major source of new antimcrobial molecules. Plants are the rich source of secondary metabolites proved therapeutic potential in many diseases. Aim of present study was to evaluate the antioxidant and antimicrobial activity of different extracts of C. sinensis. Total Phenolic content of water, hydroalcoholic, alcoholic and ethyl acetate extract was determined by fiolin- Ciocalteu method. Antioxidant activity of all extracts were determined by DPPH method. Antimicrobial activity of all extracts was determined by agar well diffusion method using gram-positive, gram negative bacteria and fungus species. Total Phenolic content was found highest in ethyl acetate fraction. Antioxidant activity of all extract was found highest at a concentration of 50 µg/ml. optimum free radical scavenging effect was found in ethyl acetate fraction. Most significant antimicrobial activity was found against A. niger, while antimicrobial activity was not found against C. albicans, E. coli and P. vulgaris. Free radical scavenging result revealed all the extract possess good antioxidant activity. Amongst all extracts, ethyl acetate extract possess optimum free radical scavenging effect Antimicrobial effect was determined by using seven strain of microorganisms. Minimum inhibitory concentration (MIC) found at 200 µg/ml. The mean diameter of zone of inhibition (ZOI) was found to be dose dependent, maximum ZOI found at 400 µg/ml against A. niger staphylococcus aureus, Bacillus sustilis, S. epidermidis. Antioxidant and antimicrobial result reveal that C. sinensis could be a good antioxidant and novel safe and effective anti microbial agent could be use in treatment of many infectious disease as a chemotherapeutic agent.

Keywords

Free radical scavenging, antioxidants, bioactive, infectious disease

Conclusion

The total phenolic content result showed that all extracts possess good phenolic content. Free radical scavenging results revealed that all the extracts possess good antioxidant activity. Amongst all extracts, ethyl acetate extract possesses the optimal free radical scavenging effect The antimicrobial effect was determined by using seven strains of microorganisms. Maximum ZOI was found at a concentration of 400 µg/ml. against A. niger, S. aureus, B. substilis, and S. epidermidis. A more potent antimicrobial effect was found in the alcoholic and ethyl acetate extracts against A. niger, and S. epidermidis than B. substilis, and S. aureus. Antimicrobial activity was not found against E. coli, C. albicans, and P. vulgaris. Antioxidant and antimicrobial results reveal that C. sinensis could be a potent antioxidant and a novel, safe, and effective antimicrobial agent that could be used in the treatment of many infectious diseases as a chemotherapeutic agent and for topical skin infections.

References

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How to cite this article

Jyotsna Anandrao Saonere, Madhuri A. Channawar, Nitin I. Kochar, Deepak Mohale and Anil V. Chandewar (2023). Evaluation of Antioxidant and Antimicrobial Potential of Herbal Phenolics. Biological Forum – An International Journal, 15(2): 1114-1118.