Effect of Different Doses of Sodium Azide on Cytogenetical and Biochemical changes in Fenugreek (Trigonella foenum graecum)

Author: Reema Srivastava, Ritu Jain, Aparna Rathore and Anugunja Verma

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Abstract

Sodium azide is a popular plant mutagen. The present study was conducted to assess the mutagenic/cytogenetic changes in root tip cells in the local variety of fenugreek (Trigonella foenum graecum). Seeds of Trigonella were treated with various concentrations of sodium azide (0.1%, 0.2%, 0.3% and 0.4%) at room temperature for 3 hours and were germinated. The treated root tip cells exhibited abnormalities such as stickiness, laggards, unequal separations and bridges. The other parameters of the study included biochemical variations like protein, carbohydrate and proline variations.

Keywords

Chromosomal abnormalities, fenugreek, stickiness, biochemical variations, chemical mutagen

Conclusion

The present study considered the mutagenic effects of sodium azide on germination percentage, chromosomal structure and biochemical variations like protein, carbohydrate and proline content in the plant- Trigonella foenum graecum. The results showed that sodium azide mutagen is effective in inducing various abnormalities such as stickiness, unequal separations, laggards and bridges in Trigonella foenum graecum, with an increase in the dose of sodium azide, which increased the number of root tip cells showing abnormal metaphase. Similarly, the increase in the proline content also shows a dose dependent increase. The study positively concluded that sodium azide could be employed to improve protein and carbohydrate content in Trigonella foenum graecum at a particular concentration. Sodium azide, as a mutagen is effective in inducing various mutagenic effects. These mutagenic effects can be used to study the nature and function of genes so as to improvement the commercial applicability of cash crops. With a future perspective, these mutagenic improvements can be brought about by the increase in the nutritional composition of the plants and also other economical aspect like increase in oil, starch, sugar content in plant to name a few.

References

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How to cite this article

Reema Srivastava, Ritu Jain, Aparna Rathore and Anugunja Verma (2023). Effect of Different Doses of Sodium Azide on Cytogenetical and Biochemical changes in Fenugreek (Trigonella foenum graecum). Biological Forum – An International Journal, 15(2): 1119-1123.