DNA Fingerprinting and Molecular Characterization of Newly Developed Maize Inbreds based on Microsatellite Markers

Author: Jyoti Sahu, Deepika Parte, Priya Gupta, Naresh Kumar Sahu, J.K. Tiwari, S.K. Sinha and Prabharani Chaudhari

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Abstract

Identification of elite and diverse parents is a critical step in the process of releasing new hybrids. DNA fingerprinting and characterization of germplasm plays a significant role in plant breeding for varietal identification where, molecular markers have proven to be very effective. The current study was performed at the Plant Molecular Biology and Biotechnology Laboratory, RMDCARS, Ambikapur (Chhattisgarh). A total of 27 SSR primers were used to check the polymorphism of eighteen newly developed maize inbreds, eight of which were found to be polymorphic and were subsequently used for DNA fingerprinting and molecular characterization. A total of 25 alleles were obtained using these polymorphic SSR primers, with an average of 3.13 alleles per primer. The PIC value for these primers ranged from 0.10 to 0.82 where, highest value obtained for primer bnlg 1867. The fingerprinting for each inbreds with unique profile identity (ID) were generated using different banding pattern and variation in allele size. These fingerprint data provide distinct allelic profiles for each inbred lines of maize. A dendrogram was also prepared for all these inbreds using the unweighted pair group method with arithmetic mean (UPGMA). It separated them into five major clusters at nearly 84% genetic similarity indicated the existence of genetic variation among the observed inbreds. This enables their further utilization for generating heterotic hybrids in future breeding programmes. Among all the inbreds studied, IAMI-57 and IAMI-43-1 were found to be more genetically diverse. The polymorphic SSR markers facilitated discrimination among genotypes and provided valuable information for future use in improvement of these genomic resources.

Keywords

DNA Fingerprinting, Microsatellite, SSR, Maize, Molecular characterization

Conclusion

The DNA fingerprinting catalogue of maize inbreds were generated using different banding pattern and variation in allele size. These fingerprint data provide distinct allelic profiles that can be used to precisely determine genotypic at any stage of crop growing cycle. Furthermore, the SSR markers based characterization facilitated discrimination among all the eighteen maize inbreds where, inbreds viz., IAMI-57 and IAMI-43-1 were found to be more genetically diverse. Though, the data were generated using a limited number of SSR primers, inclusion of more number of primers would lead to an unambiguous result.

References

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How to cite this article

Jyoti Sahu, Deepika Parte, Priya Gupta, Naresh Kumar Sahu, J.K. Tiwari, S.K. Sinha and Prabharani Chaudhari (2023). DNA Finger Printing and Molecular Characterization of Newly Developed Maize Inbreds based on Microsatellite Markers. Biological Forum – An International Journal, 15(3): 27-31.