Identification of Polymorphic SSR Markers and Diversity Analysis in a Set of Desi Chickpea Genotypes

Author: Rajesh Ningwal, Manoj Kumar Tripathi, Sushma Tiwari, Ruchi Asati, Rakesh Kumar Yadav, Niraj Tripathi and Mohammad Yasin

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Abstract

The chickpea (Cicer arietinum L.) is a staple legume crop grown in India, North Africa and the Middle East and Ethiopia. To maintain continuous production of chickpea, it is important to develop new varieties tolerant/resistant to different abiotic and biotic stresses. Therefore, it is important to identify diverse genotypes to select as parents before planning hybridization programme. So, it is necessary to analyse diversity among and between genotypes. The molecular characterization of chickpea for genetic diversity may be used for detection of diverse chickpea genotype(s). The persistence of this investigation is to examine the genetic diversity present among 57 chickpea genotypes by using SSR molecular markers. Thirteen of 33 SSR molecular markers were found to be polymorphic and produced an average of seven amplicons per primer pair. The genetic relatedness between cultivars ranged between 0.4334 to 0.8926 and the polymorphic information content arrayed between 0.3820 (STMS-11) to 0.8833 (GAA-44). Hierarchical tree data indicated 6 different clusters in a dendrogram and in bootstrapping. Overall, the study confirmed that SSRs are effective marker methods for revealing genetic diversity in chickpeas, which may be proved helpful for breeding programme such as parent selection as well as cultivar identification.

Keywords

Chickpea, Genetic diversity, SSR, PCR, scoring, Dendogram

Conclusion

High genetic diversity within a chickpea population provides an opportunity to the breeders to plant hybridization strategies for improvement of chickpea. Thirteen SSR primers were found to be polymorphic out of the 32 SSR markers used in the current study. Through the crossing of genetically diverse genotypes, traits with a wide range of allele sizes, a large number of genotype-specific alleles per locus, high polymorphic information content, and expected heterozygosity made it possible to improve yield and specific traits like heat, cold, and drought.

References

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How to cite this article

Rajesh Ningwal, Manoj Kumar Tripathi, Sushma Tiwari, Ruchi Asati, Rakesh Kumar Yadav, Niraj Tripathi and Mohammad Yasin (2023). Identification of Polymorphic SSR Markers and Diversity Analysis in a Set of Desi Chickpea Genotypes. Biological Forum – An International Journal, 15(3): 45-51.