Genetic Diversity in Turmeric (Curcuma longa L.) Genotypes using Molecular Markers

Author: Nandakumar K., Vishnuvardhana, Fakrudin B., Mohan Kumar N.V., Brijesh A. and Venkatesha J.

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Abstract

Turmeric being an economical crop hence it attracts to the farmers due to its versatile use in medicinal and spice industry. In the present study, genetic relatedness of 63 turmeric genotypes were assessed with using 37 SSR markers. A total of 90 amplicons were produced of which 61 amplicons were polymorphic accounting for 54.50 per cent and 29 amplicons were monomorphic. Among thirty seven primers pairs screened, CUMISAT 8 and CUMISAT 13 scored highest number of polymorphic alleles (5 amplicons). The cluster analysis of UPGMA reveals that all genotypes were grouped into six major clusters with four solitary clusters. The genotypes viz., TC-1, TC-2, TC-3, TC-4, TC-5, TC-8, TC-9 and TC-52 showed more similarity towards a commercially cultivated variety of Salem. Similarly, the genotypes viz., TC-59, TC-26, TC-33, TC-32 and TC-60-1 showed similarity towards released or improved variety Prathibha. The genotype TC-60-2, TC-61, TC-31 and Prabha seem to be very diverse compared to other genotypes. Hence, this molecular marker information will be a useful tool to identify the unique/diverse genotypes present in the collection. The study revealed genetic diversity and relatedness of turmeric genotypes collected by farmers with respect to the released varieties which can useful for selections in crop improvement.

Keywords

SSR, UPGMA, Amplicons, Cluster, Polymorphic and Monomorphic

Conclusion

A comparison of these values of allelic diversity among the genotypes, clearly emphasize the scope for introgression of genes through hybridization among the cultivars for increasing genetic diversity in the cultivated turmeric pool. This also reiterates the need for genetic diversity evaluation among the principal genotype classes and cataloguing them for the benefit of the future. Molecular investigation of 63 genotypes with SSR markers showed that, the genotypes viz., TC-1, TC-2, TC-3, TC-4, TC-5, TC-8, TC-9 and TC-52 showed more similarity towards commercial cultivated variety of Salem. Similarly, the genotypes viz., TC-59, TC-26, TC-33, TC-32 and TC-60-1 showed similarity towards released or improved variety Prathibha. The genotype TC-31, TC-60-2 and TC-61 were more diverse among the collected genotypes. Hence, this molecular marker information will be useful tool to identify the unique/diverse genotypes present in a collection.

References

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How to cite this article

Nandakumar K., Vishnuvardhana, Fakrudin B., Mohan Kumar N.V., Brijesh A. and Venkatesha J. (2023). Genetic Diversity in Turmeric (Curcuma longa L.) Genotypes using Molecular Markers. Biological Forum – An International Journal, 15(2): 1229-1236.