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The Study on Demographic factors and VKORC1- 1630 G>A gene Polymorphism by using PCR-RFLP in the South Indian Population

Author: Bala Tripura Sundari Ankani and R. Sivaraj

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Abstract

Deep vein thrombosis, a disease with a high mortality risk. Thomboembolic conditions require oral anticoagulants. Warfarin is used to treat DVT, everybody has a distinct range of dose requirements, and dose variation is influenced by genetic factors. Variations in the dose requirements for warfarin are primarily caused by polymorphism of the gene for vitamin K epoxide reductase complex 1 (VKORC1). The data on promoter polymorphism is limited at present Indian population. The given study is aimed to detect VKORC1 polymorphism distribution in South Indian Population. Ninety six healthy individuals are taken from south Indian population and genotyped for VKORC1-1639 G>A by PCR- RFLP Method. The VKORC1-1639G> A in the research group, a gene frequency was found. GG, GA, and AA were discovered in the range of 63.5%, 22.9%, and 13.5%. These results are consistent with how the mutation in the promoter region affects the function of vitamin K epoxide reductase. The prevalence of the wild, heterozygous, and variant homozygous alleles of VKORC1 in the South Indian community differs from that of other known ethnic groups. The therapeutic dosage of oral anticoagulants in the people of South India can be determined using this VKORC1-1639 G>A condition.

Keywords

Warfarin, Polymerase chain reaction, VKORC1 gene, polymorphism

Conclusion

Future medical practice will increasingly focus on individualized care. The frequency of VKORC1-1639 AA is 13.5%, which is close to its 23% frequency in Europeans, but it varies from that of Asians and Caucasians. The identification of these polymorphisms in patients by physicians may be useful before the start of therapy. They may use this information to determine the best course of action to reduce unpleasant drug side effects like bleeding and to enhance the curative impact.

References

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How to cite this article

Bala Tripura Sundari Ankani and R. Sivaraj (2023). The Study on Demographic factors and VKORC1- 1630 G>A gene Polymorphism by using PCR-RFLP in the South Indian Population. Biological Forum – An International Journal, 15(3): 133-137.