Evaluation of Antifungal Activities of different Clove Extracts against Sclerotium rolfsii Sacc. causing Stem Rot of Indian Bean

Author: Ranapratap Raut, Lalit Mahatma, Ravikumar Vaniya and Nikunj Khunt

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Abstract

The study evaluated the antifungal activities of different clove extracts against Sclerotium rolfsii, the causative agent of stem rot of Indian bean. The extraction of bioactive compounds from clove poses a significant challenge. To overcome this challenge, various organic solvents were employed during the extraction process. To prepare the extracts, clove powder was soaked in different solvents including acetone, propanol, mustard oil, castor oil, and water for a period of 48 hours. The in vitro evaluation was done using the Poisoned Food Technique, where a 1.0 per cent concentration of each extract was used. The results showed that propanol and acetone clove extracts showed cent per cent inhibition of S. rolfsii compared to the untreated control. Propanol alone showed 14.67 mm of mycelium growth and 83.70 per cent inhibition. This study highlights the potential of clove extracts as an alternative to chemical fungicides in managing stem rot caused by S. rolfsii in Indian bean.

Keywords

Solvents, Clove extract, Sclerotium rolfsii, in vitro

Conclusion

Organic solvents such as propanol and acetone are more effective in extracting the antifungal components present in clove compared to water and oil. Among the different solvent based clove extracts, Propanol clove extract and Acetone clove extract showed complete inhibition of S. rolfsii at 1.0 per cent concentration.

References

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How to cite this article

Ranapratap Raut, Lalit Mahatma, Ravikumar Vaniya and Nikunj Khunt (2023). Evaluation of Antifungal Activities of Different Clove Extracts against Sclerotium rolfsii Sacc. causing Stem Rot of Indian Bean. Biological Forum – An International Journal, 15(3): 273-276.