ZnO Nanoparticles bio-synthesized using Hibiscus subdariffa Leaf extract for Potential Medicinal Application in hyperbilirubinemia

Author: Niranjan Bala

Download PDF

Abstract

Zinc oxide nanoparticles (ZnO NP) were synthesized following the green route by whole leaf extract of the plant Hibiscus subdariffa. The formation of ZnO NPs was confirmed by X-ray diffraction (XRD) and UV-visible (UV-VIS) spectroscopy. The morphology and size distribution of synthesized particles were analysed by Field emission scanning electron microscopy (FESEM) and High resolution transmission electron microscopy (HRTEM). This study also provides experimental evidence for its ameliorative action against CCl4-induced hyperbilirubinemia in Swiss albino mice. Liver function enzyme assays like ALT (alanine transaminase), ALP (alkaline phosphatase), AST (aspartate aminotransferase), GGT (Gamma-glutamyl Transferase) and biochemical studies showed that altered biochemical profiles due to jaundice were restored significantly after the administration of H. subdariffa-mediated synthesized ZnO nanoconjugate over control and other treatments. Insignificant changes in hematological parameters such as Hemoglobin, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, platelets and white blood cell counts among different treatment groups suggested the bio-friendly nature of the green NPs. H. subdariffa-mediated synthesized ZnO nanoconjugate exhibits a synergistic effect of both chemically synthesized ZnO NPs and H. subdariffa herb against hyperbilirubinemia.

Keywords

Zinc oxide nanoparticles, Hibiscus subdariffa, hyperbilirubinemia, jaundice, hematological, mice

Conclusion

In summary, the result of this study demonstrates thatH. subdariffa-mediated ZnO NPs have a significant hepatoprotective effect in mice against CCl4-induced liver injury. These green synthesized NPs exhibit significant anti-hyperbilirubinemia activities in Swiss albino mice by restoring altered levels of serum bilirubin and liver function enzymes, suggesting the stabilization of hepatocytic cell membrane or recovery of injured liver cells.

References

-

How to cite this article

Niranjan Bala (2023). ZnO Nanoparticles bio-synthesized using Hibiscus subdariffa Leaf extract for Potential Medicinal Application in hyperbilirubinemia. Biological Forum – An International Journal, 15(3): 326-330.