Antioxidant and Phytochemical Analysis of Leaf Extract of Velvet Bean (Mucuna pruriens (L): An Experimental Analysis in Wistar Albino Rats

Author: Indla Ravi, Manivannan E., V Sivasankari V., Kothai Ramalingam and Regina Roy

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Abstract

Several disorders have pathophysiologies that have been linked to oxidative stress and a deficient antioxidant system. Seeds of Mucuna pruriens are used to treat and manage a range of disorders, were examined for antioxidants and phytochemicals, but there are very little or no information on the presence of phytochemicals and antioxidants in the leaf extracts of Mucuna pruriens. Based on their power to block the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, the extracts' potential as free radical scavengers were evaluated. The extract's phytochemical content was tested using ethanol and distilled water. To test the antioxidant activity of the extracts, malondialdehyde (MDA). Superoxide dismutase (SOD). and catalase (CAT) levels in the liver of albino rats treated with carbon tetrachloride (CCl4) were evaluated. A single injection of CCl4 (3 mL/kg body weight) was given to the animals after they had received the extract for six consecutive days at a dose of 400 mg/kg body weight each. The most extensively utilized antioxidant was vitamin C. The extract contained saponins, tannins, anthraquinones, terpenoids, flavonoids, and alkaloids according to a phytochemical analysis of Mucuna pruriens. Extract reduced the DPPH radical in a concentration-dependent manner and it is statistically equal to vitamin C (P >0.05) in terms of inhibition. In comparison to the positive control, the extracts markedly decreased (P 0.05) the levels of liver MDA while markedly boosting (P 0.05) the levels of SOD and CAT. These findings call attention to the extract's antioxidant capabilities and may shed light on the therapeutic usage of this plant.

Keywords

Phytochemicals, DPPH, MDA, SOD, Traditional Medicine, Mucuna pruriens, Antioxidants, CNS Activities, Adverse drug reactions, Vitamin-C, Albino rats

Conclusion

This research is important as it could lead to the development of new drugs or supplements that can help prevent or treat diseases associated with oxidative stress. Further studies are needed to fully understand the potential health benefits of these leaves and their antioxidant properties.

References

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How to cite this article

Indla Ravi, Manivannan E., V Sivasankari V., Kothai Ramalingam and Regina Roy (2023). Antioxidant and Phytochemical Analysis of Leaf Extract of Velvet Bean (Mucuna pruriens (L): An Experimental Analysis in Wistar Albino Rats. Biological Forum – An International Journal, 15(3): 343-346.