Proteomics: An Emerging Tools, Database and Technique for Understanding Biological System

Author: Amanulla Khan, R.L. Pawar, M.H. Mali, Kamlakar More and Satish V. Deore

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Abstract

Proteomics is an advance tool in protein identification, structures prediction, protein domains and post transcriptional modifications. Proteomics study have several challenges, including the complexity and dynamic nature of the proteome, technical limitations, data analysis, sample preparation, and lack of standardization. Addressing these challenges will require continued innovation and collaboration among scientists, technologists, and bioinformaticians to advance the field of proteomics and realize its full potential in advancing our understanding of biological systems. Various technologies are developed and evolved time to time accordingly to the requirement and availability of the design experiments. Including traditionally used 2DE, immunological to advance modern tandem MS, with more data accuracy and resulted inferences. Information’s, reports and data of proteome from these technologies result to build a specific, organized, and formed databases. These biological databases are evolved from primary to secondary databases and many special, accordingly to their specific features. Databases are Improve and develop with relations and collaboration to others database in various forms and formats. These all techniques and databases are having various latest improvements and additions in recent, which help in high level of protein study to understanding biological system level.

Keywords

2DE, tandem MS, Proteome, Proteomics, Biological Databases, and Biological System

Conclusion

Recent developments in protein research databases such as SWISS-PROT, CluSTr, Universal Protein Resource, UniRef, PRIDE, InterPro, ProToMap, International Protein Index, RESID, and PHYTOPROT, have expanded the scope of protein research. These databases provide crucial information on protein structure and function, and have become a valuable resource for protein identification, prediction, and annotation. UniProt has been recently updated to provide more detailed information on protein isoforms and their functions, as well as protein-protein interactions (The UniProt Consortium 2021). In addition, InterPro has been updated to provide more accurate and comprehensive protein domain predictions, while RESID has been updated to include new post-translational modifications (Mitchell, 2019, Huang, 2020). Moreover, new databases have emerged, such as the Protein Data Bank (PDB), which provides high-resolution 3D structures of proteins, and the Human Protein Atlas (HPA), which provides detailed information on the expression and localization of human proteins (Uhlén, 2015 and Burley, 2021). The availability of these databases has led to significant advancements in protein research, and has accelerated the discovery of new drug targets and biomarkers for diseases. In conclusion, these databases continue to play an important role in facilitating protein research and advancing our understanding of the biological world (Burley, 2021).

References

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How to cite this article

Amanulla Khan, R.L. Pawar, M.H. Mali, Kamlakar More and Satish V. Deore (2023). Proteomics: An Emerging Tools, Database and Technique for Understanding Biological System. Biological Forum – An International Journal, 15(3): 511-523.