Evaluation of different DNA Extraction Methods from Blood for LAMP Reaction

Author: Sindhoora Divakar, Dhanalakshmi H., Sandeep N., Shrikrishna Isloor, Rashmi R., Ananda K.J. and Manjunatha Reddy

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Abstract

DNA-based methods are becoming familiar techniques for the detection and identification of specific organisms. These methods rely on the ability to detect specific DNA sequences in the genome of target organisms. The main objective of the study is to facilitate DNA extraction from the blood at the field level. In the present study, simple methods of DNA extraction from blood were optimized and compared with kit method to assist LAMP (Loop mediated isothermal amplification). The boiling method was the simplest and cheapest when compared to all the four methods. But the quantity of DNA was highest in case of DPK (Detergent proteinase K) method. Apart from these methods, the filter paper method is also a simple, time saving method which can also be considered as a good alternative.

Keywords

DNA, LAMP, Boiling, DPK method, filter paper

Conclusion

As a result, a higher yield was obtained with the DPK method when compared to Qiagen kit method. Qiagen kit method is the most expensive of all the four methods, whereas DPK method is the most laborious and time consuming of all the four methods. Boiling method provides reasonable amounts of DNA that are usually enough to be used in LAMP. It is also very easy and inexpensive. The use of dried blood spots on filter paper becomes an attractive alternative to blood collection in tubes for LAMP analysis. Sample transport and storage for dried blood spots is easy and less expensive.

References

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How to cite this article

Sindhoora Divakar, Dhanalakshmi H., Sandeep N., Shrikrishna Isloor, Rashmi R., Ananda K.J. and Manjunatha Reddy (2023). Evaluation of Different DNA Extraction Methods from Blood for LAMP Reaction. Biological Forum – An International Journal, 15(3): 530-533.