In vitro Regeneration of Plantlets from Nodal Explants of Aristolochia bracteata Retz. – An Important Medicinal Plant

Author: Ramsiya A. and A.S. Rubin Jose

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Abstract

Natural propagation of Aristolochia species is hindered by its low viability of seeds. Aristolochia bracteata Retz. is one of the major species of the genus that faces challenges with its seed viability. So, its multiplication through the in vitro culture is one of the best ways to overcome such challenges. Accordingly, an efficient regeneration protocol through in vitro direct organogenesis was developed for the valuable medicinal plant Aristolochia bracteata Retz. using nodal segments as explants. The present study established a reproductive protocol for the conservation via in vitro culture as well as its secondary metabolite enhancement. Varying concentrations of plant growth regulators of cytokinin (Benzyl adenine, Kinetin and Thidiazuron) and auxin (Indole-3-acetic acid, Indole-3-butyric acid and 1-Naphthaleneacetic acid) were used for micropropagation in Murashige and Skoog medium. The plant showed multiple roots and shoots at lower concentrations of Indole-3-acetic acid (0.9 mg/l) and Benzyl adenine (0.7 mg/l) respectively. Maximum shoots were attained in Thidiazuron, but all the cultures showed anomalous growth. Shoot with large-sized leaves were obtained in kinetin. Rooted plantlets were transferred to the natural environment after appropriate acclimatization.

Keywords

BA, Benzyl adenine; KIN, Kinetin; TDZ, Thidiazuron; IAA, Indole-3-acetic acid; IBA, Indole-3-butyric acid; NAA, 1-Naphthaleneacetic acid; MS, Murashige and Skoog

Conclusion

The low viability of seed impedes the natural propagation of the plant. As a part of plant propagation, the present study exemplifies a triumphant development of in vitro propagation of Aristolochia bracteata Retz. It is known that lower concentration of IAA and BA induced multiple roots and shoots respectively. BA is strongly recommended than kinet into obtain the highest percentage of regeneration and the highest number of shoots without any anomalies. Plantlet with elongated shoots were given from medium amended with a combination of KIN and IBA. However, TDZ produces multiple stunted shoots in which the regenerated plantlet is not taken for further in vitro culture studies. In conclusion, the micropropagation method is valuable for conserving Aristolochia bracteata Retz., a medicinally important plant. Retardation in its natural propagation can be overcome through this regeneration protocol, and it enhances the overall conservation of the species.

References

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How to cite this article

Ramsiya A. and A.S. Rubin Jose (2023). In vitro Regeneration of Plantlets from Nodal Explants of Aristolochia bracteata Retz. – An Important Medicinal Plant. Biological Forum – An International Journal, 15(3): 547-552.