Bixin-a Diapocarotenoid as Inhibitor of Glutathione Peroxidise (GP×1): A Potential Target for Cancer Treatment: An in silico Study

Author: Kumaraswamy Aavunuri, G. Shyam Prasad and T. Christopher Reuben

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Abstract

Cancer is a significant health problem and leading cause of deaths worldwide. These cells are gaining resistance against different chemotherapeutic agents. Hence, exploring new targets is a continuous process. Cancer cells produce greater amount of reactive oxygen species (ROS) and for their progression and metastasis upregulation of antioxidant enzymes are required. Glutathione peroxidase (GPX) is a selenocystic –containing peroxidase enzyme which defends mammalian cells against oxidative damage by reducing hydrogen peroxides and organic hydroperoxides. The enzyme GP×1 is over expressed in various cancer cells what eliminates reactive oxygen species (ROS), weakens apoptosis and induce drug resistance promoting cancer cell survival. Exploring GP×1 inhibitors and their applications for cancer therapy is mandatory. Hence, in the present investigation GP×1 inhibition potentials of the Red pigment Bixin was investigated In silico. An advanced autodock 4.2 version was used for the study. Bixin has shown strong inhibition potentials of GP×1compared to the standard drug. Furter, DNA binding studies were also performed and strong binding of Bixin to the DNA was recorded compared to the standard drug -5-Fluoro Uracil. Hence, from the binding scores, hydrogen bond interactions with the receptors, it can be concluded that the compound Bixin may be developed as a drug for treating cancers which inhibits GP×1 enzyme. However, further in vitro and in vivo investigations are required to develop Bixin as a final drug.

Keywords

In silico, GP×1, Bixin, Autodock, cancer, DNA binding

Conclusion

In the present investigation, Glutathione peroxide (GP×1), a vital enzyme involved in minimizing oxidative stress to the cancer cells is inhibited by a red pigment produced by plant seeds which leads to death of cancer cells. Similarly, in order to find other possible targets where Bixin binds and prevents cancer cell proliferation DNA binding studies were performed and strong binding of Bixin with DNA was found when compared to the standard drug -5-fluoro uracil. Binding of Bixin to DNA interferes with the protein synthesis and replication leading to death of the cancer cells. However, further investigation in In vitro and in vivo are required to confirm.

References

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How to cite this article

Kumaraswamy Aavunuri, G. Shyam Prasad and T. Christopher Reuben (2023). Bixin-a Diapocarotenoid as Inhibitor of Glutathione Peroxidise (GP×1): A Potential Target for Cancer Treatment: An in silico Study. Biological Forum – An International Journal, 15(3): 660-664.