Comparative Genomics Analysis of some Selected Pseudomonas Strains having Biocontrol, Plant Growth Promoting and Bioremediation activities using Bioinformatic Tools

Author: Mehzabin Rehman, Sabnoor Yeasrin Jyoti, Preetom Regon, Subrat Kakati, Piyush Kumar Mishra and Bhaben Tanti

Download PDF

Abstract

Pseudomonas that are associated with plants, often found living as parasites or saprophytes on the surfaces or inside plant species. Such species of Pseudomonas associated with plants may promote growth of plants by eliminating pathogenic microbes thereby synthesizing plant growth stimulating hormones and enhancing disease resistance in plants, biological control of plant pathogens and bioremediation. The present investigation was conducted with an aim to study comparative genomic studies of 14 Pseudomonas strains having biocontrol, PGPR and bioremediation activities keeping P. fluorescens as the reference strain. The study revealed that these strains are somewhat nearly related strains based on the various parameters undertaken, and therefore can be used collectively. With the increasing availability of sequences, the complexity of genome alignment and analysis is growing drastically with which the computational requirements of the EDGAR 2.0 and Mauve 2.3.1 have risen considerably over the past decade which supports an easy, user-friendly interface of evolutionary relationships in terms of gene order thereby gaining new biological insights of differential gene content.

Keywords

Pseudomonas, biocontrol, PGPr, bioremediation, genomics, EDGAR 2.0, MAUVE 2.3.1

Conclusion

The study undertaken elucidated the comparative genomics between different Pseudomonas strains that have biocontrol, plant-growth promoting and bioremediation activities. The study revealed how closely or distantly the species are related in terms of their gene orders that enumerated genome rearrangements, orthologous regions of one genome to that of another in course of their evolution. The observations also revealed that some of the biocontrol and plant-growth promoting Pseudomonas are somewhat closely related in terms of their gene orders, and hence, can be used in either way i.e. in pathogenic and remediation projects. Thereby, the softwares used supports a quick and user-friendly survey of evolutionary relationships between microbial genomes and simplifies the process of obtaining new biological insights into their differential gene content.

References

-

How to cite this article

Mehzabin Rehman, Sabnoor Yeasrin Jyoti, Preetom Regon, Subrat Kakati, Piyush Kumar Mishra and Bhaben Tanti (2023). Comparative Genomics Analyses of some Selected Pseudomonas strains having Biocontrol, Plant Growth Promoting and Bioremediation activities using Bioinformatic Tools. Biological Forum – An International Journal, 15(6): 01-16.