Design of a Validated HPLC Methodology for the Measurement of Linoleic Acid and Beta Sitosterol in Solanum nigrum

Author: Arpan Chakraborty, Arka Bhattacharjee, Baishakhi Mondal, Manas Chakraborty, Goutam Mukhopadhyay and Alpana Majumder

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Abstract

Solanum nigrum, a member of the Solanaceae family, has a long history of medicinal use. It has immunomodulatory, anti-ulcerogenic, hepatoprotective, and anti-hyperlipidemic effects. It has been used to treat measles, malaria, and cancer. The plant contains alkaloids, coumarins, flavonoids, tannins, saponins, proteins, carbohydrates, glycosides, and phytosterols. S. nigrum is notable for its linoleic acid content, and the plant itself has a higher lipid content compared to other plants. S. nigrum is an essential ingredient in various herbal and Ayurvedic preparations. The objective of the study was to develop a reliable HPLC method for simultaneous quantification of linoleic acid and beta-sitosterol in S. nigrum extract berries. One potential challenge of the study could be optimizing the separation and detection parameters for accurate quantification of linoleic acid and beta-sitosterol. A validated RP-HPLC method was developed in accordance with ICH guidelines. The separation was achieved using an isocratic approach on a C18 column with specific conditions. The developed method demonstrated satisfactory linearity, with correlation coefficients of 0.9982 and 0.9971 for LA and BS, respectively. The LOD and LOQ values indicated the sensitivity of the method for both compounds. Overall, the HPLC method proved to be efficient, precise, and reproducible, offering advantages such as speed, cost-effectiveness, and the ability to maintain isocratic conditions throughout the analysis. The study contributes to the field by providing a validated HPLC methodology for accurately quantifying linoleic acid and beta-sitosterol in S. nigrum, which can aid in quality control and therapeutic applications.

Keywords

Solanum nigrum, linoleic acid, beta-sitosterol, HPLC, validation

Conclusion

A precise and reliable HPLC method has been successfully developed and validated for the quantification of two active compounds, including LA and BS, in SNB extract. This method is deemed suitable for routine quality control analysis of SNB extract. The analytical conditions employed in the method offer excellent resolution for the separation of LA and BS, ensuring accurate quantification. The validation of the method was performed following the guidelines set by the ICH, and it successfully met all the crucial parameters, including robustness. The findings of this study are expected to serve as a valuable quality control reference for the standardization of these specific components in formulations or herbal raw materials.

References

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How to cite this article

Arpan Chakraborty, Arka Bhattacharjee, Baishakhi Mondal, Manas Chakraborty, Goutam Mukhopadhyay and Alpana Majumder (2023). Design of a Validated HPLC Methodology for the Measurement of Linoleic Acid and Beta Sitosterol in Solanum nigrum. Biological Forum – An International Journal, 15(6): 86-92.