Callus Induction and Plant Regeneration of Spilanthes acmella M. an Ornamental and Medicinal Herb

Author: Nasrin Nasr

Download PDF

Abstract

Spilanthes acmella M is an important ornamental cum medicinal plant. In order to investigate the effects of different concentrations, different explants, and combinations of growth regulators on callus induction and plant regeneration of Spilanthes acmella M. Leaf explants were inoculated onto MS medium supplemented with different concentrations of α-naphthalene acetic acid (NAA), 2,4-dichlorophenoxy acetic acid (2,4-D), indol acetic acid (IAA) and benzyl adenine (BA) alone and auxins in combinations with BA for callus induction. Callus initiation was observed in all media evaluated and the highest callus formation (0.29 ± 0.03 mg) was obtained from explants cultivated in the presence of IAA alone at 10 µM in nodal explant or 15 µM BA + 5 µM 2,4-D (0.33 ± 0.039 mg) in combination. Maximum shoot induction (81 %) and highest number of shoot per callus was obtained from callus induced on 15 µM BA + 5 µM 2,4-D three weeks after transferring the callus to a MS medium supplemented wi

Keywords

Callus induction, plant regeneration, shoot induction, Spilanthes acmella.

Conclusion

In this study, a protocol was established for callus induction and plant regeneration from the organogenic calli. In conclusion, calli were successfully induced from all three explants and the procedure for plant regeneration from induced calli has been developed. The analysis of data indicates that proliferation of callus in media with different growth regulator with different concentration of phytohormones was varied and the callus induction was mainly depended on growth regulator type and present of cytokinin in media containing auxin. However if multiplication of selected clones for further studies is required, leaf explants could be cultured on MS medium supplemented 15 µM BA + 5 µM 2,4-D. Organogenesis in S. acmella M. proved to be controlled by the ratio of cytokinin /auxin in the medium. Our result showed that the medium supplemented with only BA induced shoots from compact callus in less frequency whereas higher percentage of shoot proliferation obtained with callus explants

References

-

How to cite this article

Nasrin Nasr (2016). Callus Induction and Plant Regeneration of Spilanthes acmella M. an Ornamental and Medicinal Herb , Biological Forum – An International Journal 8(1): 509-517.