Estimation of Biochemical changes in wheat (Triticum spp.) due to Spot Blotch Diseases (Bipolaris sorokiniana Sacc.) by using different Treatments

Author: Vishwa Vijay Raghuvanshi, Subhash Chandra, Shyam Narayan Patel, Suraj Kumar Patel, Shubham Patel, Abhishek Singh and Prabha Siddharth

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Abstract

Bipolaris sorokiniana (Sacc.) Shoemaker, a hemi biotrophic, phytopathogenic fungus, is the cause behind spot blotch disease. Warmer and more humid regions around the world are more prone to the spot blotch disease of wheat. Spot blotch (Bipolaris sorokiniana) symptoms start out as little, brown lesions that spread throughout the leaf and get larger as the disease progresses. Total phenolic contents and Phenylalanine ammonia-lyase that have been well-documented to play a significant role in disease resistance in a number of pathosystems, inhibiting the colonization of plant tissue. Different intervals of hours after inoculation (hai) have an impact on the total phenolic content (TPCs) and phenylalanine ammonia-lyase (PAL) in wheat leaves. Inoculation at various intervals (00 hai, 24 hai, 48 hai, and 72 hai) affects the amount of TPCs and PAL in wheat leaves. Maximum phenolic contents (134 mg/g, 156 mg/g, 221 mg/g, and 82 mg/g at 00 hai, 24 hai, 48 hai, and 72 hai, respectively) were recorded in T4 (seed treatment with Pseudomonas fluorescens @ 10g/kg of seed + 2 foliar spray garlic clove extract), while lowest phenolic contents were observed in T9 (untreated). The T4 treatment plot (seed treatment with Pseudomonas fluorescens @ 10g/kg of seed + 2 foliar spray garlic clove extract) had the highest phenylalanine ammonia-lyase concentrations (0.191, 0.214, 0.374, and 0.042 at 00 hai, 24 hai, 48 hai, and 72 hai, respectively), whereas the T9 control plot (untreated) had the lowest concentrations.

Keywords

Total phenolic contents, Phenylalanine ammonia-lyase, Spot blotch, Bipolaris sorokiniana and Hours after inoculation

Conclusion

1. In the present investigation, botanical and bio-agent treated plots recorded higher total phenolic contents (TPCs) and phenylalanine ammonia lyase (PAL) as compared to chemically treated and untreated plots. 2. Different time intervals after inoculation have an impact on the total phenolic content (TPCs) and phenylalanine ammonia-lyase (PAL) in wheat leaves. 3. Total phenolic Content (TPC) and Phenylalanine Ammonia-lyase (PAL) were measured at various time points after inoculation (hai). They considerably increased with passing time, from 24 to 48 hours after inoculation, and then decreased at 72 hours after inoculation. 4. Maximum phenolic contents (134, 156, 221 and 82 at 00 hai, 24 hai, 48 hai and 72 hai respectively) was recorded in T4 (seed treatment with Pseudomonas fluorescens @ 10g/kg of seed + 2 foliar spray garlic clove extract) and lowest phenolic content was recorded in T9 (untreated). 5. Highest phenylalanine ammonia-lyase (0.191, 0.214, 0.374 and 0.042 at 00 hai, 24 hai, 48 hai and 72 hai respectively) was noted in T4 (seed treatment with Pseudomonas fluorescens @ 10g/kg of seed + 2 foliar spray garlic clove extract) and lowest was found in T9 control plot (untreated).

References

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How to cite this article

Vishwa Vijay Raghuvanshi, Subhash Chandra, Shyam Narayan Patel, Suraj Kumar Patel, Shubham Patel, Abhishek Singh and Prabha Siddharth (2023). Estimation of biochemical changes in wheat (Triticum spp.) due to spot blotch diseases (Bipolaris sorokiniana Sacc.) by using different treatments. Biological Forum – An International Journal, 15(6): 192-198.