Antimicrobial and Anti – inflammatory activities of Plectranthus amboinicus Leaf extract on Silkworm Pathogen Streptococcus pyogenes SA 1

Author: S. Saranya, R. Raja Jeya Sekar, A. Harish and S.M. Vijila

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Abstract

To study the antimicrobial effect of herbal plant Plectranthus amboinicus on the silkworm pathogen Streptococcus pyogenes SA1. The herbal plant P. amboinicus - leaf was extracted by using known solvents such as acetone, methanol and distilled water and the antimicrobial effect of these extracts were studied against S. pyogenes SA 1 by agar well diffusion method. The phytochemical constituents of these extracts were also determined and GC-MS analysis was done. The phytochemical analysis of acetonic extract of P. amboinicus - leaf was revealed the presence of alkaloids, flavonoids, coumarins, steroids, glycosides, carbohydrates, tannins and saponins. The acetone and the methanol extracts of P. amboinicus - leaf showed minimum inhibitory activity 10 ± 0.03 mm and 3 ± 0.08 mm respectively in 0.5µl concentration and maximum inhibitory activity 18 ±0.15 mm, 10± 0.01 mm respectively against bacterial pathogen S. Pyogenes SA 1. The distilled water extract of P. amboinicus - leaf showed no inhibitory activity and maximum inhibitory activity 2 ±0.004 mm in 2.0 µl concentration on S. Pyogenes SA 1. The GC-MS analysis of the acetone extract of P.amboinicus – leaf was showed the presence of 18 compounds. The acetone and methanol extract of P. amboinicus – leaf showed remarkable antimicrobial activity against silkworm pathogen S. pyogenes SA 1 and the GC-MS analysis of the acetonic extract of P. amboinicus – leaf showed the presence of various antimicrobial compounds. In vitro and in vivo tests have to be carried out to confirm these findings.

Keywords

Plectranthus amboinicus, chemical composition, GC-MS, antimicrobial activity

Conclusion

P. amboinicus is an important aromatic medicinal herb packed with many bioactive constituents and nutrients, which are important for maintaining good health. The biological properties are attributed to the occurrence of a wide range of bioactive compounds in P. amboinicus- leaf extracts which possess promising anti-microbial effects. The GC-MS studies of the acetonic extract of P. amboinicus – leaf was showed various compounds. Further study is required to find out the accurate compound responsible for the plants medicinal value. The major components of nonadiyne are used in biological activities.

References

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How to cite this article

S. Saranya, R. Raja Jeya Sekar, A. Harish and S.M. Vijila (2023). Antimicrobial and Anti – inflammatory activities of Plectranthus amboinicus Leaf extract on Silkworm Pathogen Streptococcus pyogenes SA 1. Biological Forum – An International Journal, 15(6): 199-205.