Alkaline Proteases Producing Bacillus species Isolated from River (Aram) Side Soil

Author: Rajendrabhai Daulatbhai Vasait

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Abstract

In the current work, bacteria having the capacity to synthesise extracellular proteases were screened out. From rhizosphere soil and soil samples taken from the nearby riverfront in Satana, Dist. Nashik, Maharashtra, an effort was undertaken in order to obtain protease-producing bacterial strains. On a skim milk agar plate, ten strains showed zones of clearance around colonies. The isolates were biochemically characterised by tests such as indole, methyl red, Vogues Proskauer, citrate utilization, detection of enzyme production assays for amylase, oxidase, urease, catalase, and the capacity to hydrolyse starch. Because of protein hydrolysis, isolate RDV 01 had the greatest zone of clearance. The enzyme produced was an extracellular, alkaline protease, according to the characterization. The isolate RDV 01 was identified on the basis of morphological, biochemical characteristics, 16S rRNA sequencing, and a phylogenetic tree. After studying the morphological and biochemical characters, the isolated organism was identified as a member of the genus Bacillus, and NCBI blast and phylogenetic analysis confirmed that the isolate RDV 01 may be related to species JSG1 with 100% homology as revealed. Industrially important alkaline proteases production from bacterial sources has been evaluated in this study.

Keywords

Alkaline proteases, Bacillus, Skim milk agar, Proteolysis

Conclusion

In this present study, 10 bacterial isolates were examined for the zone of clearance around colonies on skim milk agar plates. The bacterial isolate RDV 01 exhibited a considerably maximum zone of hydrolysis of protein. The Bacillus species RDV 01 was assessed for its ability to produce proteolytic enzymes and be active in an alkaline environment. In the present study, we successfully isolated and identified Bacillus strain RDV 01 producing alkaline proteases, which may prove their applicability for various purposes after extensive study.

References

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How to cite this article

Rajendrabhai Daulatbhai Vasait (2023). Alkaline Proteases Producing Bacillus species isolated from River (Aram) Side Soil. Biological Forum – An International Journal, 15(6): 306-310.