Identification of viruses Naturally infecting Patchouli (Pogostemon cablin (Blanco) Benth.) in India

Author: Humaira Bano and Jawaid A. Khan

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Abstract

Patchouli (Pogostemon cablin) is considered one of the most important aromatic medicinal plants. A number of viruses are known to attack patchouli plant, causing mosaic symptoms and significantly reduce the quality and quantity of patchouli production. To prevent crop loss or minimise crop damage, accurate viral detection and identification are required before developing virus control methods. However only a handful of information is available in India for their identification. This study aims to identify and characterise the viruses associated with mosaic disease of patchouli using RT-PCR. Infected plant samples were collected from different patchouli cultivated states in India such as Assam, Karnataka and Palampur. RT-PCR employing pair of degenerated primers, specific to both potyvirus and fabavirus, yielded DNA fragments representing Peanut stripe virus and Broad bean wilt virus 2, respectively. Furthermore, RT-PCR using the designed primers specific to 3' end of PStV yielded amplicon that represent nuclear inclusion b protein (partial), coat protein (CP, complete) and 3' UTR. RT-PCR with the designed primers specific to BBWV2 produced an amplicon that encompases the large and small CP (partial). The PCR amplicons were subjected to nucleotide sequencing. The Phylogenetic analysis clustered Indian PStV isolates together with those from Taiwan, Vietnam and Thailand in a single clade. According to phylogenetic analysis of BBWV2, Indian isolates were clustered with Chinese and German isolates. Thus, RT-PCR assay developed here can act as a diagnostic tool for detection and epidemiological investigation of PStV and BBWV2.

Keywords

Broad bean wilt virus 2, Peanut stripe virus, Patchouli, Phylogenetic, RT-PCR

Conclusion

PStV (potyvirus) and BBWV2 (fabavirus) have been identified as the viruses associated with the mosaic disease of patchouli plants in India based on the highest nucleotide identities of CP gene sequence and their phylogenetic relationship. Phylogenetic analysis of PStV isolates under study showed close resemblance with Taiwan, Vietnam and Thailand. The phylogenetic analysis of BBWV2 showed its close resemblance with China and Germany isolates. Consequently, the present study reveals the virus diversity, subgrouping, evolutionary history within the patchouli viruses in India.

References

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How to cite this article

Humaira Bano and Jawaid A. Khan (2023). Identification of viruses naturally infecting Patchouli (Pogostemon cablin (Blanco) Benth.) in India. Biological Forum – An International Journal, 15(6): 549-558.