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Isolation and Biochemical Characterisation of Food Born Pathogens Listeria Monocytogenes from Beverages

Author: Keshamma E.

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Abstract

Listeriosis in human is very often associated with very high mortality, and clinical cases of listeriosis in humans caused by Listeria monocytogenes. Hence, current study was undertaken with the prime objective of isolation and characterization of food borne pathogens L. monocytogenes from different soft drinks sold at local market outlets in the city of Chikkaballapura, Karnataka. Samples comprising of different types of locally soft drinks were collected aseptically and subjected to serial dilution and inoculated to nutrient agar medium, Palcam selective agar base medium and Tryptic soy broth. The growth with the typical colony characteristics were further identified up to species level based on their morphological and biochemical characteristics. The bacteria isolated from different soft beverages was found to be gram positive bacilli, non-motile, showed positive for catalase, methyl red, and Voges Proskauer, CAMP tests. Whereas, isolated strains were negative for oxidase, indole, and citrate utilization tests. Based on the morphological and biochemical characterization isolated strain was identified as L. monocytogenes. In conclusion, results of the presented study suggested that L. monocytogenes is prevalent in beverages samples and sheds new light on the growth of food pathogens and spoilage microbes in a variety of beverages that are increased in popularity worldwide. These findings could be considered in estimating the risk associated with the production and consumption of beverages.

Keywords

Beverages, L. monocytogenes, Morphology, Biochemical tests, Softdrinks

Conclusion

The findings of the study lead to new light on the growth of food pathogens and spoilage microbes in a variety of beverages that are growing in popularity across the globe and suggested that L. monocytogenes is common in beverage samples. These results might be taken into account when calculating the risk connected to beverage production and consumption.

References

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How to cite this article

Keshamma E. (2023). Isolation and Biochemical Characterisation of Food Born Pathogens Listeria Monocytogenes from Beverages. Biological Forum – An International Journal, 15(6): 599-602.