Screening and Identification of Ligninolytic Enzyme Producing Bacteria for the Degradation of Polyethylene Glycol

Author: R. Janani and R. Amutha

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Abstract

In the commercial poultry industry, antibiotics are applied for various intents, including feed as growth promoters that elaborate antimicrobial resistance (AMR). We're facing challenge to disrate AMR without affecting the growth performance. The goal of the current study was to find out how Shatavari (Asparagus racemosus) root meal supplementation affects broiler chicken growth and haematological characteristics. At the Livestock Farm Complex, C.V.Sc.&A.H., Kumarganj, Ayodhya, U.P., the study was conducted for 42 days. One control group and three treatment groups, each with 50 chicks, were given the following treatments: TC (0% Shatavari root powder), T1 (0.5% Shatavari root powder), T2 (1% Shatavari root powder), and T3 (1.5% Shatavari root powder). A completely randomized design (CRD) was used to divide each group into two replicates of 25 chicks each. At the conclusion of the trial, T2 had the largest body weight (2547.76g), followed by T1, TC, and T3. The TC group exhibited the highest overall feed intake (4339.64g), followed by T1, T2, and T3. T2 and T1 had the lowest FCR (1.58), which was followed by T3 and TC. In comparison to the TC, the mean Hb concentration of the T2, T3, and T1 groups of birds was considerably greater (p 0.05). Birds in the T2 group had considerably greater PCV% than those in the TC, T1, and T3 groups. In comparison to the treatment groups, the TLC of TC was considerably (p 0.05) lower. For the groups TC, T1, T2, and T3, the H:L ratios were determined to be 0.47, 0.49, 0.54, and 0.52, respectively. Therefore, it can be inferred that broiler chickens growth performance and haematological parameters are much higher when 1% Shatavari (Asparagus racemosus) root powder supplementation is added to their commercial diet.

Keywords

Polyethylene glycol (PEG), polyethylene glycol (PEG), lignin peroxidase, laccase, Pseudomonas stutzeri

Conclusion

The ligninolytic isolates demonstrated the ability to degrade polyethylene glycol (PEG). The ligninolytic isolates, which possess enzymes capable of breaking down lignin, showed promise in breaking down PEG as well. The degradation of PEG by ligninolytic isolates is significant as it offers a potential solution for the remediation of PEG-contaminated environments. The ability of these isolates to break down PEG suggests that they have the potential to be utilized in bioremediation strategies or waste treatment processes involving PEG-contamination. Further research is needed to fully understand the mechanisms by which ligninolytic isolates degrade PEG and optimize the degradation process. Overall, the findings of this study highlight the potential of ligninolytic isolates as a natural and efficient means for the degradation of polyethylene glycol, contributing to the development of eco-friendly solutions for PEG waste management.

References

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How to cite this article

R. Janani and R. Amutha (2023). Screening and Identification of Ligninolytic Enzyme Producing Bacteria for the Degradation of Polyethylene Glycol. Biological Forum – An International Journal, 15(6): 701-706.