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Influence of Growth Regulators on Organogenesis of Unshiu mandarin

Author: Influence of Growth Regulators on Organogenesis of Unshiu mandarin

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Abstract

Virus-free plants is very important in citrus breeding program. This study was conducted to evaluate the Influence of growth regulators on Unshiu mandarin (Citrus unshiuowari) seed rganogenesis at in vitro conditions in 2014, in a completely randomized design with three replications. In this study treatments, including 9 levels of hormones (control, BA-1, BA-2, GA-1, GA-2, IBA-0.25, IBA-0.5, NAA-0.25, NAA-0.5), respectively. In order to measure traits during development stages of randomly sampling and measurement were conducted and analyzed. Most of the highest (3.25 and 3.05 cm)to 2 mg/l GA and 1 mg/l GA respectively, the maximum length of the root (7.12 and 7.02 cm) to 0.5 mg/l NAA and IBA, the largest number of root (9.75) to 0.5 mg per liter of IBA, the largest number of shoot (1.5) corresponds to 2 mg/l and BA, the highest percentage of callus induction (70%) of the NAA was 0.5 mg .

Keywords

Unshiu mandarin, Callus induction, GA, Germination.

Conclusion

Virus-free plants is very important in citrus breeding program. This study was conducted to evaluate the Influence of growth regulators on Unshiu mandarin (Citrus unshiuowari) seed rganogenesis at in vitro conditions in 2014, in a completely randomized design with three replications. In this study treatments, including 9 levels of hormones (control, BA-1, BA-2, GA-1, GA-2, IBA-0.25, IBA-0.5, NAA-0.25, NAA-0.5), respectively. In order to measure traits during development stages of randomly sampling and measurement were conducted and analyzed. Most of the highest (3.25 and 3.05 cm)to 2 mg/l GA and 1 mg/l GA respectively, the maximum length of the root (7.12 and 7.02 cm) to 0.5 mg/l NAA and IBA, the largest number of root (9.75) to 0.5 mg per liter of IBA, the largest number of shoot (1.5) corresponds to 2 mg/l and BA, the highest percentage of callus induction (70%) of the NAA was 0.5 mg .

References

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How to cite this article

Influence of Growth Regulators on Organogenesis of Unshiu mandarin (2016). Influence of Growth Regulators on Organogenesis of Unshiu mandarin , Biological Forum – An International Journal 8(1): 522-525.