Characterizing Phytate Hydrolyzing Fungal isolate using ITS Region Profiling

Author: Suchita P. Bharambe and Swati A. Peshwe

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Abstract

Along with the conventional methods of identification, the advance genome sequencing and computational methods are advantageous for identification of various lives. Enormous databases of nucleotide sequence are now available as a result of advance genome sequencing and computational methods. Such databases can be retrieved, assembled and analyzed for identification of various cells. Identification of a phytate hydrolyzing fungal isolate fsp-4 as Aspergillus terreus was carried out on the basis of macroscopic, microscopic and molecular characterization using ITS sequences. Dealing with complex and diverse fungal communities in various environmental samples was the challenge in characterizing fungal isolate using ITS region profiling, also identifying specific phytate-hydrolyzing fungi amidst the vast array of microbial species. Even with these challenges, the study contributed significantly to our understanding of phytate hydrolyzing fungi, shedding light on their role in nutrient cycling and their potential applications in agriculture, biotechnology, and environmental remediation. The use of ITS region profiling allows for a more comprehensive and rapid identification of these fungi, enabling researchers to explore their diversity and ecological significance more effectively. The findings from this study may pave the way for developing sustainable agricultural practices and optimizing phytate degradation in various industrial processes, leading to enhanced nutrient availability and reduced environmental impact.

Keywords

Genome sequencing, computational methods, Aspergillus terreus, molecular characterization, ITS sequences

Conclusion

The phytate hydrolyzing fungus was identified as Aspergillus terreus using ITS region sequencing. Thus, utilization of ITS region profiling as a molecular tool proved effective in identifying and characterizing phytate-hydrolyzing fungus. This approach enabled a more comprehensive understanding of the fungal populations involved in phytate hydrolysis, offering potential applications in feed industry.

References

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How to cite this article

Suchita P. Bharambe and Swati A. Peshwe (2023). Characterizing Phytate Hydrolyzing Fungal isolate using ITS Region Profiling. Biological Forum – An International Journal, 15(5a): 06-11.