Standardization of Sterilization Protocol for Explants of Melia dubia Cav.–An Important Short Rotation Tree

Author: Bodalkar Sarita, Prajapati R.K., Mannade Ajit and Vishwakarma S.P.

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Abstract

Soft wood forest species are under extreme pressure all over the world as a result of growing demand to meet out industrial requirements as a raw resource. One of these is the rapidly expanding demand of Melia dubia. Large-scale plants cannot be produced through means of seed for this species. Therefore, the aim of the current research work was to create a protocol for M. dubia multiplication using the tissue culture approach. The key to establishing an aseptic culture is to standardize sterilization procedure, in view of this present investigation was conducted. Nodal explants, taken from mature M. dubiatrees, were subjected to experiments to see how well they could be disinfected. Maximum explants survival percentage 86.66% was obtained with 0.01% HgCl2 for 5 min followed by 73.3% percent with 1% NaOCl for 3 min. Contamination per cent was also least with these two treatments 13.33 % contamination was recorded with 0.01 % HgCl2 for 5 min and 20 % contamination was recorded with 1% NaOCl for 3 min. Overall 0.01% HgCl2 for 5 min produced the best results for sterilization of nodal explants of Melia dubia. The developed sterilization protocol has potential to be successfully employed for micropropagation of M. dubia.

Keywords

M. dubia, Sterilization, Response, Explant, Fedility, survival

Conclusion

There were differences between the explant responses to various type & concentrations of sterilization agents. For nodal explants sterilization in M. dubia 0.01%HgCl2 was found best & effective sterilizing agent. Over sterilization increased tissue mortality of explants and hence to overcome to this problem, optimized concentration of sterilizing agents & duration is required. In case of M. dubia nodal explants surface sterilization with 0.1% (v/v) Polyoxyethylene sorbitan monooleate (Tween - 20; Himedia, India) for 15 min., then 0.1% (w/v) solution of Bavistin (Carbendazim 50% WP- a systemic fungicide) for 30 minutes followed by treatment with 0.01% HgCl2 for 5 min. proved most effective for maximum survival percentage, minimum contamination and no drying of explants, followed by treatment with 1% NaOCl for 3min (Table 1 and Fig. 1).

References

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How to cite this article

Bodalkar Sarita, Prajapati R.K., Mannade Ajit and Vishwakarma S.P. (2023). Standardization of Sterilization Protocol for Explants of Melia dubia Cav.–An Important Short Rotation Tree. Biological Forum – An International Journal, 15(5a): 22-25.