Effect of different Combinations of Plant Growth Regulators on Sugarcane Shoot Multiplication

Author: Krishanu, R.S. Sengar, Mukesh Kuma, Vaishali, M.K. Yadav and L.K. Gangwar

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Abstract

For the production of disease-free plant on large scale plant tissue culture technique is the best for utilization in vitro. The current experiment used the different plant growth regulators for the development of sugarcane plantlets using plant tissue culture technique. About 86% of the world's total sugar needs are satisfied by sugarcane. India is the world's second-largest producer of sugar. Sugarcane is traditionally replicated vegetatively, utilising setts as seed rather than real seeds. Low rates of multiplication and disease susceptibility are typically the outcome of this method of sugarcane propagation. Techniques for growing plant tissues provide a reliable and effective answer to this problem. The production of pathogen-free plants and a significant improvement in plant quality are both being explained by tissue culture. Auxins are used for roots, whereas cytokinin is used for shoot initiation and multiplication. In the current study, Murashig and Skoog (MS) medium were employed. Different sixteen combinations (15+Control) of modified MS media were used for in-vitro shoot multiplication in both sugarcane genotypes (Cos 13235 and Co 15023). The study showed that the combination of sterilant in sequence of labolene (2%) for 10 min, Bavistin (0.1%) for 5 min, HgCl2 (0.1%) for 90 seconds and Ethanol (70%) for 1 min was found to be best for surface sterilization. Minimum growth of explants was observed in media which is devoid of any growth regulator. It is concluded that both the genotypes have shown higher growth and shoot regeneration in T11 containing (0.5 BAP + 0.5 Kinetin mg/L). Some challenges in the present study are 1. Ensuring pathogen-free and disease-free plant production through tissue culture technique on a large scale requires meticulous and consistent sterilization procedures to prevent contamination. 2. Finding the optimal combination of growth regulators in the culture medium for shoot multiplication can be challenging, as it involves extensive experimentation and precise control of hormonal concentrations. 3. Achieving consistent and efficient growth and shoot regeneration across different sugarcane genotypes can be difficult due to variations in their inherent characteristics and responses to the tissue culture conditions.

Keywords

Sugarcane, Plant growth regulators, Culture medium, BAP and Kinetin

Conclusion

A well-standardized protocol for in-vitro shoot tip culture of sugarcane genotypes Cos 13235 and Co 15023 is done by treating with different hormonal combinations. The best growth was observed in the MS medium supplemented with a hormonal concentration of 0.5 mg/L BAP + 0.5 mg/L Kinetin in both of the sugarcane genotypes Cos 13235 and Co 15023. On the basis of results obtained in this study, it can be concluded that the developed protocol for shoot tip culture can be used for in-vitro shoot tip culture of sugarcane in commercial level, it also shows relative advantage such as taking short duration for shoot multiplication, disease free culture plants.

References

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How to cite this article

Krishanu, R.S. Sengar, Mukesh Kuma, Vaishali, M.K. Yadav and L.K. Gangwar (2023). Effect of different Combinations of Plant Growth Regulators on Sugarcane Shoot Multiplication. Biological Forum – An International Journal, 15(5a): 171-176.