Production and Optimization of Biofuel from Lignocellulosic waste using natural Bacterial consortia

Author: V. Aruna, S. Jeyabharathi and N. Jeenathunisa

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Abstract

Biofuels are produced from microorganisms as a by product of metabolic activity of organic or cellulosic waste products. The target was achieved the low cast renewable source to fulfil the current requirement. In order to be considered a bio fuel the fuel must contain over 80% renewable materials. Ethanol is and highly demand bio fuel made from feed stocks such as different food crops and food crop wastes after the pre-treatment. We explore such the bacterial consortium that couples the high cellulosic activity of the mixed culture of bacteria to ferment hexose and pentose sugars to ethanol. The present study we analysed the three bacterial consortia for the degradation of the agro cellulosic waste such as turmeric stem, bamboo leaf, orange peel and Jack fruit inner peel by submerged fermentation for cellulose production. In quantification of biofuel, bacterial species Enterobacter sp (SA-02), was most efficient and produced maximum amount of bio ethanol on the sixth day of incubation it showed 2.04g/l ethanol. Further findings showed that Bacillus sp (SA-05), Pseudomonas sp (SA-07) played on efficient role in 5.60 g/l and 4.03 g/l respectively and bacterial consortia showed the maximum level of enzyme activities and pattern of ethanol production. The optimization study for ethanol production makes clear to the fact that high temperature and pH, only 350c and pH 8 were considered optimum. At the optimum substrate concentration was found to be 10.25 g/l after 6 days of incubation which summed as a total yield of 0.132 g of ethanol per g of dried Jack fruit inner peel. This current study evaluates the potential of turmeric stem, bamboo leaf, orange peel and Jack fruit inner peel as a production. These existing studies evaluate the potential jack fruit inner peel as a probable substrate for ethanol production. We found that Natural bacterial consortia showed the efficient production is required to make the process industrially feasible.

Keywords

Ethanol, bacterial consortia, fermentation, optimization, Enzyme activity

Conclusion

Bio ethanol is one of the most suitable and cost effective bio fuel. In our current finding we gained bio ethanol from four orange peel, turmeric stem, bamboo leaves and jack fruit inner peel collected from local market around Trichy. With a isolation of bacterial consortium containing Enterobacter sp (SA-02), Bacillus sp (SA-05) and Pseudomonas sp (SA-07), the ethanol was produced by fermentation process. After 10 days we could gained10.25± 0.34 G/L ethanol from jack fruit inner peel as a best substrate and maintaining PH of 6 and of 30°C. We could infer that more concentrated form of ethanol could be obtained by distillation. The more concentrated ethanol could be used as a bio fuel, which releases no toxic gases out in the environment. As a result, the findings of this study show cases the effects of different pre-treatment, processing and optimization methods for the production of bio ethanol from variety of agro wastes.

References

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How to cite this article

V. Aruna, S. Jeyabharathi and N. Jeenathunisa (2023). Production and Optimization of Biofuel from Lignocellulosic waste using natural Bacterial consortia. Biological Forum – An International Journal, 15(5a): 247-252.