Combat of Bacteriocin Producing Lactiplantibacillus pentosus LMEM1001 isolated from Neera with Bactericidal activity against MRSA and VRSA Strains

Author: Narmada Talloli, Vandana Rathod, Roopa Niroli, Ravi M., Shajji Dahwood, Dattu Singh, Krishna Rayudu and Sujata Hoshmani

Download PDF

Abstract

Antimicrobial resistance accelerates due to overuse and indiscriminate use of antibiotics by all living creature. Indiscriminate use of antibiotics and other chemical compounds leads to several side effects and results in increased antibiotic resistance day by day. The emergence of antibiotic-resistant bacterial strains, including Extended-Spectrum Beta-Lactamase (ESBL), Methicillin-Resistant Staphylococcus aureus (MRSA), and Vancomycin-Resistant Staphylococcus aureus (VRSA), has become a significant global health concern. To address this issue, alternative antimicrobial agents are being explored, and bacteriocins produced by lactic acid bacteria are gaining attention due to their potential as natural alternatives to conventional antibiotics. Bacteriocins are ribosomally synthesized antimicrobial peptides produced by bacteria, harmless to human and more effective for gastroinstestinal tract infections. Study investigates the bactericidal activity of bacteriocins from by Lactiplantibacillus pentosus LMEM1001 (VRN-32) isolated from Neera a traditional probiotic drink. Bacteriocin production was confirmed by well diffusion assays. Bacterioicin from VRN-32 was purified by ammonium sulphate precipitation, and RP-HPLC. Partially Purified bacteriocin was stable against heat and extreme pH. Inhibitory effects of bacteriocin found to be stable over a broad temperature 80°C and pH 8.0 Bacterioc in was stable with Lysozyme and resistant to proteinase-K. Stability of bacterioic in was maximum with surfactants such as Tween-80 and Triton X-100, where as SDS and β-mercaptoethanol found to decrease its stability. Bacteriocin from VRN-32 showed promising antimicrobial activity against Methicillin Resistant Staphylococuss aureus (ATCC- 33591) and Vaccomycin Resistant Staphylococuss aureus VRSA stains isolated from hospital acquired infections. Lantibiotic isolated from probiotic drink neeera was partial purified and showed promising activity against emerging Super Bugs and the probiotic found to be an excellent alternative to hazardous antibiotics.

Keywords

Antimicrobial resistance, Neera, MRSA, VRSA

Conclusion

Antimicrobial peptides called bacteriocins produced by bacteria and have the ability to either kill or stop the growth of other bacteria. Bacteriocins hold great promise as a new class of antibiotics and food preservatives, but more research is needed to fully understand their potential and to develop effective methods for their production and application. With the increasing threat of antibiotic resistance a new viable alternative is necessary. Our bacteriocins isolated from L. pentosus LMEM1001 have broad inhibitory action against gram positive, gram negative and fungal pathogens and it is stable at high temperature and low pH, which can be used in biopreservation and medical fields as bacteriocin are effective against MRSA,VRSA strains. Hence, bacteriocin produced by L. pentosus LMEM1001 can represent a promising alternative that could help combat the spread of infectious diseases and improve food safety and control diseases caused due to pathogens with nil side effects.

References

-

How to cite this article

Narmada Talloli, Vandana Rathod, Roopa Niroli, Ravi M., Shajji Dahwood, Dattu Singh, Krishna Rayudu and Sujata Hoshmani (2023). Combat of Bacteriocin Producing Lactiplantibacillus pentosus LMEM1001 isolated from Neera with Bactericidal activity against MRSA and VRSA Strains. Biological Forum – An International Journal, 15(5a): 338-344.